Interactive multiple gene expression map for the brain

大脑的交互式多基因表达图

• 批准号: 6478294
• 负责人: KIMINOBU SUGAYA
• 金额: $ 18.09万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2006-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6478294
• 关键词: Internet; aging; autoradiography; bioengineering /biomedical engineering; bioimaging /biomedical imaging; brain; brain imaging /visualization /scanning; brain mapping; brain morphology; computer human interaction; computer system design /evaluation; gene expression; histochemistry /cytochemistry; image processing; in situ hybridization; information dissemination; information systems; laboratory rat; male; sectioning

DESCRIPTION (provided by applicant): Current molecular biological techniques allow us to visualize semi-quantitative levels of gene expression in situ. For example, autoradiographical image data from in situ hybridization histochemistry (ISHH) provides a wealth of information, which if made readily available could be beneficial for neuroscience researchers. However, in most cases due to the scope of their particular research interests this ISHH data has been widely overlooked by many researchers. Thus, much of the gene expression data tends to get discarded or at best becomes unavailable for future review by other researchers. For example, one researcher may have vested interests in the gene expression of brain type nitric oxide synthase (bNOS) in cholinergic nuclei, and other researchers may have interests in the serotonergic nuclei. Until this proposal, there has been no considerable effort to facilitate the sharing of such bNOS gene expression data amongst researchers. In essence, for their particular research interests, these investigators will need to repeat the same bNOS ISHH for their publications, time and time again. In order to eliminate such progress-retarding redundancy, we propose a data sharing system (interactive Multiple Gene Expression Map, IMGEM) that will be available as an interactive brain map of gene expression. This interactive tool will provide benefits essential for continuing to make great strides in discovery and mapping of gene expression by neuroscientists. We will employ the technological advantages of electronic databasing by creating a series of brain atlases as follows: 1) IMGEM will contain 2D images of all brain sections with multiple levels of resolution, 2) by the 2D and 3D image analysis, IMGEM will facilitate the comparison of multiple gene expressions and morphological structures (Niss1-stain), 3) by three dimensional reconstruction of the image data, IMGEM will allow for free rotation of the 3D image and virtual-sectioning of the brain will be possible in any desired plane, 4) IMGEM will include a server-side, graphical interface discussion board (or discussion forum) capability, which will be capable of receiving responses or input from IMGEM users in real-time; and as an additional benefit, IMGEM will be readily edited and updated to reflect the real-time input of online users, 5) IMGEM will be seamlessly integrated with other currently available online databases and hyperlinks to other data resources on the Internet will be highlighted and easily accessible via IMGEM's user-friendly design and navigation. The long-term goal of this current proposal is to gain further insights from the information available (data in the present and future) for brain gene expression mapping; and in so doing, to seek to better apply this collective knowledge for our continued understanding of normal and diseased human brain function. Our laboratory has been providing prominent ISHH data in serial brain sections with special expertise in the combination of ISHH and immunohistochemistry techniques in the same brain section. The applicant has exhibited skilled experience in creation of a commercial software package for multi dimensional data analysis, construction of relational database system on network, computer aided manipulation of imaging data and in programming of HTML, DHTML, VRML, JAVA, CGI and Perl scripts, languages necessary to accomplish the scope of the IMGEM project. Thus, we believe that our laboratory is uniquely, qualified to pursue this current proposal.

描述（由申请人提供）：当前的分子生物技术 允许我们在原位可视化基因表达的半定量水平。为了 例如，来自原位杂交的自显影图像数据 组织化学（ISHH）提供了大量信息 可用的可能对神经科学研究人员有益。但是，大多数 案件由于其特定研究兴趣的范围，这是ISHH数据 许多研究人员广泛忽视了。因此，大部分基因 表达数据往往会被丢弃，或者充其量是不可用的 其他研究人员的未来审查。例如，一个研究人员可能有 一氧化氮合酶的基因表达中的既得兴趣 （BNO）在胆碱能核中，其他研究人员可能对 血清素能核。在此提案之前，没有大量 努力促进共享此类BNO基因表达数据之间的共享 研究人员。本质上，对于他们的特定研究兴趣，这些 调查人员将需要重复同一bnos ishh的出版物， 一次又一次。为了消除这种进步的冗余， 我们提出了一个数据共享系统（交互式多基因表达图， imgem）将作为基因表达的互动脑图提供。 这种互动工具将为继续制作带来必不可少的好处 神经科学家在发现和映射基因表达方面的大步进展。 我们将利用电子数据库的技术优势 创建一系列的大脑地图集如下：1）IMGEM将包含2D图像 在所有分辨率多个级别的大脑部分中，2）由2D和3D 图像分析，IMGEM将促进多个基因的比较 表达式和形态结构（NISS1迹），3）由三个 图像数据的维度重建，IMGEM将允许免费 3D图像的旋转和大脑的虚拟剖面是可能的 在任何所需的平面中，4）IMGEM将包括服务器端图形接口 讨论委​​员会（或讨论论坛）功能将能够 实时接收IMGEM用户的响应或输入；作为一个 其他好处，IMGEM将很容易进行编辑和更新，以反映 在线用户的实时输入，5）IMGEM将与 其他当前在线数据库以及其他数据的超链接 Internet上的资源将被突出显示，并可以轻松地通过 IMGEM的用户友好设计和导航。这个长期目标 当前的建议是从可用信息中获得进一步的见解 （目前和未来的数据）用于脑基因表达映射；这样 做，寻求更好地将此集体知识应用于我们的持续 了解正常和患病的人脑功能。 我们的实验室一直在串行脑部提供突出的ISHH数据 具有ISHH和免疫组织化学结合的特殊专业知识 同一大脑部分的技术。申请人表现出熟练 创建用于多维的商业软件包的经验 数据分析，网络，计算机上关系数据库系统的构建 辅助操纵成像数据和HTML，DHTML，VRML的编程 Java，CGI和Perl脚本，需要完成范围所必需的语言 IMGEM项目。因此，我们认为我们的实验室是独特的，有资格 追求当前的建议。