In Vitro Analysis of Embryonic Inner Ear Cells

胚胎内耳细胞的体外分析

• 批准号: 6504714
• 负责人: LYNNE M. BIANCHI
• 金额: $ 10万
• 依托单位: OBERLIN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-05 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6504714
• 关键词: cell differentiation; cell migration; cell morphology; cochlea; ear hair cell; early embryonic stage; embryo /fetus cell /tissue; histogenesis; immunocytochemistry; laboratory mouse; labyrinth; scanning electron microscopy; tissue /cell culture; tissue mosaicism; vestibular apparatus

DESCRIPTION (provided by applicant): The goal of the current project is to determine the cell-mediated signals that direct cell patterning of the developing inner ear using an in vitro assay. Preliminary data from this lab revealed that dissociated, embryonic inner ear cells reassociate into three distinct aggregate types in vitro, yet only one aggregate type contains sensory hair cells. These hair cells are surrounded by supporting cells, similar to the pattern observed in vivo. How this precise reassoication occurs is unknown. Aim 1 will further characterize the aggregation of inner ear cells in vitro by testing the hypotheses that both cochlear and vestibular hair cells reaggregate and that this reaggregation is limited to early stages of development. Aims 1 and 2 will test two alternative mechanisms by which inner ear cells organize into the precise mosaic. Aim 2 will test the hypothesis that individual hair cells differentiate, then migrate within the cultures to aggregate into specific patterns using cell recognition cues. Aim 3 will test the hypothesis that individual hair cells remain undifferentiated until aggregates are formed, then use the notch signaling pathways to establish cell fate. Together, these experiments will provide information regarding the mechanisms used for organizing normal auditory and vestibular epithelia and may provide insight into means of treating congenital anomalies of the inner ear.

描述（由申请人提供）：当前项目的目的是确定使用体外测定的细胞介导的信号，该信号导致发育中的内耳的细胞模式。该实验室的初步数据表明，分离的，胚胎的内耳细胞在体外重新定位为三种不同的聚集体类型，但只有一种骨料类型包含感觉毛细胞。这些毛细胞被支撑细胞包围，类似于体内观察到的模式。这种确切的放松如何发生是未知的。 AIM 1将进一步表征内耳细胞在体外的聚集，通过测试耳蜗和前庭毛细胞会重新凝结的假设，并且这种重新凝结仅限于发育的早期阶段。目标1和2将测试两种替代机制，通过这些机制将内耳细胞组织到精确的镶嵌物中。 AIM 2将测试单个毛细胞分化，然后在培养物中迁移以使用细胞识别提示汇总成特定模式的假设。 AIM 3将检验以下假设：在形成聚集体之前，单个毛细胞保持未分化，然后使用Notch信号通路来建立细胞命运。总之，这些实验将提供有关用于组织正常听觉和前庭上皮的机制的信息，并可以提供有关治疗内耳先天异常的手段的见解。