NMR OF TRANSITION BETWEEN DIMER & TETRAMER OF EK18, MUTANT OF TRP REPRESSOR

二聚体之间转变的 NMR

• 批准号: 6298209
• 负责人: CATHERINE A ROYER
• 金额: $ 0.75万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6298209
• 关键词: biological products; biomaterials; biomedical resource; nuclear magnetic resonance spectroscopy; proteins

EK18 is a mutant of trp repressor and is cal a "super-repressor" since it represses the transcription of genes responsible for typtophan synthesis at a lower level of tryptophan molecules in the cell. Its molecular weight is about 12.5 kDaltons per monomer and it exists primarily as a dimer. However, when the salt concentration is lowered, or the protein concentration is increased, oligomerization occurs between dimers. The protein-protein interaction seems to play a role in the mechanism of repression. In addition to the corepressor concentration, this oligomerization may act to fine tune the gene regulation. Several studies have been done using fluorescence spectroscopy to investigate the oligomerization, but no structural studies have been done to date. NMR studies of this oligomerization will tell how the two dimers interact (i.e., whether the tetramerization is specific or nonspecific, or whether is it symmetric or unsymmetric).

EK18 是色氨酸阻遏蛋白的突变体，被称为“超级阻遏蛋白” 因为它抑制负责基因的转录 色氨酸合成的色氨酸分子水平较低 细胞。 每个单体的分子量约为 12.5 kD 主要以二聚体形式存在。 然而，当盐浓度为 降低，或蛋白质浓度增加，寡聚化 发生在二聚体之间。 蛋白质与蛋白质之间的相互作用似乎起作用 镇压机制中的作用。 除了辅阻遏物 浓度，这种寡聚化可能会起到微调基因的作用 规定。 已经利用荧光进行了多项研究 光谱法研究低聚，但没有结构 迄今为止已经进行了研究。 这种低聚反应的 NMR 研究 将讲述两个二聚体如何相互作用（即，是否 四聚化是特异性的还是非特异性的，或者是否是对称的 或不对称）。