STABALIZED LIPID PLATFORMS FOR PROTEIN IMMOBOLIZATION

用于蛋白质固定化的稳定脂质平台

基本信息

批准号：
6164758
负责人：
JOHN C CONBOY
金额：
$ 2.16万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-03-01 至
项目状态：
未结题

项目摘要

The goal of the proposed research is to prepare and characterize novel polymeric phospholipid bilayer assemblies as substrates for protein attachment. Immobilization of protein receptors on the bilayer surface has important practical applications for the development of new biosensors utilizing the bound protein as the biologically selective recognition element. The implementation of lipid bilayers as substrates for protein immobilization and subsequent use as biosensors requires that the chemical and structural properties be improved while maintaining the low nonspecific binding properties of lipid bilayer. The strategy to be investigated here is the lateral polymerization of the phospholipid bilayer in conjunction with site directed immobilization of proteins on the surface. The proposed research will involve preparation and characterization of novel tethered planar polymer phospholipid bilayers. The stability of the prepared cross- linked polymer phospholipid bilayers will then be assessed by solubility and optical measurements. The polymer lipid bilayers will subsequently be utilized as substrates for site directed protein immobilization. The proposed research will attempt to discern if the low nonspecific adsorption characteristic of fluid lipid bilayer are preserved upon cross-linking of the lipid bilayer and covalent attachment to the substrate. The resulting assembly should result in a stable protein- lipid assembly with an oriented presentation of the adsorbed protein film with maximum retention of protein structure and function.

拟议研究的目标是制备和表征新型聚合磷脂双层组件作为蛋白质附着的底物。将蛋白质受体固定在双层表面对于开发利用结合蛋白质作为生物选择性识别元件的新型生物传感器具有重要的实际应用。将脂质双层作为蛋白质固定的底物并随后用作生物传感器需要改善化学和结构特性，同时保持脂质双层的低非特异性结合特性。这里要研究的策略是磷脂双层的横向聚合以及表面上蛋白质的定点固定。拟议的研究将涉及新型束缚平面聚合物磷脂双层的制备和表征。然后通过溶解度和光学测量来评估所制备的交联聚合物磷脂双层的稳定性。聚合物脂质双层随后将用作定点蛋白质固定的底物。拟议的研究将尝试辨别流体脂质双层的低非特异性吸附特性是否在脂质双层交联和共价附着到基质上时得以保留。所得的组装应产生稳定的蛋白质-脂质组装体，并定向呈现吸附的蛋白质膜，最大限度地保留蛋白质结构和功能。