GABAA RECEPTORS IN DEVELOPMENTAL EPILEPTOGENESIS

发育性癫痫发生中的 GABAA 受体

• 批准号: 6394116
• 负责人: Amy R. Brooks-Kayal
• 金额: $ 26.91万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394116
• 关键词: GABA receptor; child (0-11); clinical research; dentate gyrus; developmental neurobiology; generalized seizures; granule cell; human subject; laboratory rat; neural conduction; partial seizure; pilocarpine; receptor expression; single cell analysis; temporal lobe /cortex disorder; voltage /patch clamp

DESCRIPTION: (adapted from applicant's abstract) Previous studies have shown that the GABA-A receptor subunit composition changes with development and with certain seizure states such as status epilepticus (SE). The current proposal tests the hypothesis that the slowly developing temporal lobe epilepsy (TLE) often seen after SE is due to changes in the subunit composition. To test this hypothesis, the PI uses the pilocarpine-induced SE model in developing rats. Outcome measures of the GABA-A receptor are patch-clamping for conductance and pharmacological response changes and single-cell RNA analysis of the recorded cell. Similar studies will also be done in epileptic and non-epileptic pediatric populations. Finally, the effect of GABA active drugs on GABA receptor development will be examined.

描述：（改编自申请人的摘要）先前的研究表明 GABA-A受体亚基的组成随发育和 某些癫痫发作状态，例如癫痫持续状态（SE）。当前的建议 检验以下假设，即缓慢发展的颞叶癫痫（TLE） 通常在SE之后看到的是由于亚基组成的变化。测试这个 假设，PI在发育中的大鼠中使用毛果果诱导的SE模型。 GABA-A受体的结果度量是贴片夹的电导和 记录的药理响应变化和单细胞RNA分析 细胞。还将在癫痫和非癫痫症中进行类似的研究 小儿种群。最后，GABA活动药物对GABA的影响 将检查受体开发。