NEURAL PLASTICITY/VASCULAR REMODELING AFTER MINISTROKE

小中风后的神经可塑性/血管重塑

• 批准号: 6351856
• 负责人: LING WEI
• 金额: $ 24.35万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6351856
• 关键词: angiogenesis; antipyrine; autoradiography; brain circulation; brain metabolism; carbohydrate metabolism; cerebral artery; deoxyglucose; deoxyuridine; dextrans; drug metabolism; glucose metabolism; integrins; laboratory rat; microcirculation; neural plasticity; somesthetic sensory cortex; stroke

A new directly observable model for targeted strokes in adult Wistar rats will be used to test recovery of function in somatosensory cortex through restored microcirculation and neural plasticity. This unique model uses direct mapping of functional neural activity and blood flow as case studies in the same rats by videomicroscopy before, acutely after, and 30 days after ligation of 3-5 local branches of the middle cerebral artery supplying whisker barrel cortex. The hypotheses to be tested are: Growth of arteriolar collaterals is necessary for restoration of blood flow. Angiogenesis produces new intraparenchymal capillaries. Neural responses and neurovascular coupling reappear by 30 days. Activation by single whisker stimulation can occur in novel cortical areas after that whisker's barrel was lost in the infarct. Reorganized or new neuroanatomical projections are correlated with new response areas. Angiogenesis and neural reorganization are interrelated in recovery from stroke. The methods include functional mapping by intrinsic optical signals, evoked potentials, identification and measurements of arteriolar collaterals with time, local arteriovenous transits of fluorescein, BrdU labeling of vascular proliferation, autoradiography for local blood flow and glucose utilization, and mapping small infarcts and neuroanatomical projections by postmortem histology. This rodent model tests mechanisms for recovery of local blood flow and of neural function that bear on human stroke rehabilitation.

一个新的直接可观察模型，用于成人Wistar中的目标中风 大鼠将用于测试体感皮质中功能的恢复 通过恢复的微循环和神经可塑性。 这个独特 模型使用功能神经活动和血流的直接映射 作为以前通过视频显微镜在同一大鼠中的案例研究，敏锐的 在3-5个中间的3-5个本地分支结束后30天之后 脑动脉提供晶须桶皮层。 要测试的假设是：小动脉占领的生长是 恢复血流所必需的。血管生成会产生新的 核内毛细管。 神经反应和神经血管 耦合再出现30天。单晶须刺激激活 在那个晶须的枪管丢失之后，可能发生在新型皮质区域 在梗塞中。 重组或新的神经解剖学预测是 与新的响应区域相关。 血管生成和神经 重组与中风的恢复相关。方法 包括固有光信号的功能映射，唤起 小动脉侧支的电势，鉴定和测量 随着时间的流逝，荧光素的局部动静脉转移，BRDU标记 血管增殖，用于局部血流的自显影术和 葡萄糖利用，并绘制小梗塞和神经解剖学 事后组织学的预测。 这种啮齿动物模型测试机制 为了恢复局部血流和神经功能的恢复 人类中风康复。