MUSCLE REFLEX CONTROL OF THE CIRCULATION

肌肉反射控制循环

• 批准号: 6390011
• 负责人: Lawrence I Sinoway
• 金额: $ 18.79万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-05 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6390011
• 关键词: adenosine; afferent nerve; biological models; blood circulation; blood flow measurement; blood pressure; cats; electromyography; electrophysiology; exercise; heart rate; hydrogen ions; lactates; microdialysis; neuromuscular system; phosphates; potassium; reflex; sympathetic nervous system

The long-range goals of the PI are to evaluate the mechanisms that regulate blood flow delivery during exercise. The short-range goal of this proposal is to examine the role muscle metabolites play in evoking the exercise pressor reflex. Specifically, prior studies examining the metabolic engagement of this skeletal muscle reflex have not characterized interstitial concentrations of many of the putative metabolic stimulants. This remains an important concern since the free endings of the afferent nerves in question reside within the muscle interstitium. In this proposal, a decerebrate, unanesthetized, cat model will be used to further characterize the effects of interstitial metabolites on the muscle reflex. These experiments utilize microdialysis techniques to characterize interstitial concentrations of a number of potential metabolic stimulants. Specifically, we will utilize microdialysis to measure the interstitial concentrations of lactate, hydrogen ion, potassium, phosphate and adenosine. These measurements will be performed under two separate experimental conditions: 1) during a rhythmic contraction paradigm illicited with a variable amplitude and frequency stimulator as blood pressure, heart rate, renal sympathetic nerve activity and muscle sympathetic nerve activity are measured, and 2) in a separate group of experiments, the circulation to the cat hindlimb will be isolated and perfused with many of these various potential stimulants as interstitial measurements are made and reflex responses are measured. We believe these studies may help us further understand the role various metabolites play in evoking the muscle reflex. Novel components of the work described include the use of microdialysis to measure multiple interstitial metabolites, the use of a decerebrate preparation, the use of rhythmic contraction paradigms, and the use of an isolated hindlimb perfusion system.

PI的远程目标是评估调节运动过程中血流递送的机制。 该提案的短期目标是研究肌肉代谢物在唤起运动式训练反射中的作用。具体而言，先前研究了这种骨骼肌反射的代谢参与的先前研究未表征许多假定的代谢兴奋剂的间隙浓度。这仍然是一个重要的问题，因为相关神经的自由结局位于肌肉间质中。 在此提案中，将使用杂交，未经麻醉的CAT模型进一步表征间质代谢产物对肌肉反射的影响。 这些实验利用微透析技术来表征许多潜在的代谢刺激剂的间隙浓度。具体而言，我们将利用微透析来测量乳酸，氢离子，钾，磷酸盐和腺苷的间隙浓度。 这些测量将在两个独立的实验条件下进行：1）在节奏收缩范式中以可变的振幅和频率刺激物数字化，作为血压，心率，肾脏交感神经活动和肌肉交感神经活动和肌肉交感神经活性，以及​​在一个单独的实验中，在猫的循环中，将在各种刺激中进行互动，并在猫的循环中进行两组，这些刺激均与这些触发器相关，这些刺激是这些刺激的刺激，这些刺激是这些刺激。进行测量并测量反射响应。 我们认为这些研究可能有助于我们进一步了解各种代谢物在唤起肌肉反射中的作用。 所描述的工作的新成分包括使用微透析来测量多种间质代谢产物，使用杂交脑剂制剂，使用节奏收缩范式以及使用孤立的后肢灌注系统。