SHORT TERM SYNAPTIC PLASTICITY OF CORTICAL INTERNEURONS

皮质中间神经元的短期突触可塑性

基本信息

批准号：
6330394
负责人：
KATRINA M MACLEOD
金额：
$ 0.52万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-12-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/6330394
关键词：
anticholinergic agent cerebral cortex cholinergic agents cholinergic receptors gamma aminobutyrate glutamate receptor interneurons laboratory rat neural inhibition neural plasticity neuropharmacology norepinephrine serotonin synapses voltage /patch clamp

项目摘要

Short-term synaptic plasticity has a profound effect on the function of the neural circuit in which it is embedded. Previous work in the Nelson lab has characterized the short-term plasticity in the rat visual cortex using a quantitative (parametric) method, using trains of evoked stimuli delivered over a range of physiological frequencies. extend results previously obtained for pyramidal cells to a characterization of the short-term plasticity of inhibitory inputs to inhibitory interneurons by making whole-cell voltage-clamp recordings of nonpyramidal neurons in layers 2/3 in slices of rat neocortex. These inhibitory interneurons will be characterized and identified physiologically and morphologically. Our goals are: 1) Characterize the interneuron-interneuron synaptic plasticity of evoked IPSCs by extracellular or intracellular presynaptic stimulation; 2) Determine if the synaptic plasticity is differentially expressed depending on the interneuron subtype of the presynaptic or postsynaptic neuron; and 3) Investigate whether the interneuron-interneuron synaptic plasticity can be modified by neuro-modulatory agents such as acetylcholine. By investigating the cholinergic modulation of cortical synaptic plasticity, these results may have implications for behavioral and cognitive brain function, and the neurological disorders, such as Alzheimer's disease, which may be related cholinergic dysfunction.

短期突触可塑性对其所嵌入的神经回路的功能具有深远的影响。尼尔森实验室之前的工作使用定量（参数）方法，使用一系列生理频率传递的诱发刺激来表征大鼠视觉皮层的短期可塑性。通过对大鼠新皮质切片中 2/3 层的非锥体神经元进行全细胞电压钳记录，将先前获得的锥体细胞结果扩展到抑制性中间神经元的抑制性输入的短期可塑性的表征。这些抑制性中间神经元将从生理学和形态学上进行表征和鉴定。我们的目标是：1）表征通过细胞外或细胞内突触前刺激诱发的IPSC的中间神经元-中间神经元突触可塑性； 2）确定突触可塑性是否根据突触前或突触后神经元的中间神经元亚型而有差异表达； 3）研究中间神经元-中间神经元突触可塑性是否可以通过神经调节剂（例如乙酰胆碱）来改变。通过研究皮质突触可塑性的胆碱能调节，这些结果可能对行为和认知大脑功能以及神经系统疾病（例如阿尔茨海默病）产生影响，这可能与胆碱能功能障碍有关。