MINORITY PREDOCTORAL FELLOWSHIP PROGRAM

少数族裔博士前奖学金计划

• 批准号: 6322308
• 负责人: ROBERT W MAITTA
• 金额: $ 3.73万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-11-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6322308
• 关键词: SCID mouse; Streptococcus pneumoniae; Streptococcus pneumoniae vaccine; antibody titering; antigen antibody reaction; antiidiotype antibody; bacterial virus; enzyme linked immunosorbent assay; human tissue; immunocytochemistry; immunodeficiency; monoclonal antibody; passive immunization; vaccine development

The long-term objective of the study is the development of new approaches to pneumococcal vaccination that will provide better protection to patients with impaired immunity. Streptococcus pneumoniae is a pathogen responsible for great morbidity and mortality in different populations. The increase of antibiotic resistance to this and other bacteria, and the fact that current pneumococcal vaccines are not completely effective accentuate the need for better vaccines. Increased susceptibility to pneumococcal infection is particularly evident in immune impaired groups such as HIV+ individuals, newborns, bone marrow recipients, and the elderly, though S. pneumoniae causes disease in both immunocompetent and immunocompromised individuals. This proposal will use the phage-displayed technology to identify peptide mimotopes that bind to antibodies reactive with the capsular polysaccharide of serotype 8 pneumococcus (PPS-8). The peptides will be used as vaccine targets to determine if they elicit protective antibody responses in experimental pneumococcal infection. SCID-hu mice will be utilized to study the antibody responses to the peptides. Thus, human antibody responses can be studied. In addition, the reagents generated can be used to study the specificity of vaccine elicited antibodies in different patient groups.

该研究的长期目标是开发肺炎球菌疫苗接种的新方法，为免疫力受损的患者提供更好的保护。 肺炎链球菌是一种在不同人群中造成高发病率和死亡率的病原体。 对这种细菌和其他细菌的抗生素耐药性的增加，以及当前肺炎球菌疫苗并非完全有效的事实，都凸显了对更好疫苗的需求。 尽管肺炎链球菌会在免疫功能正常和免疫功能低下的个体中引起疾病​​，但在免疫受损群体（例如 HIV+ 个体、新生儿、骨髓接受者和老年人）中，对肺炎球菌感染的易感性增加尤其明显。 该提案将使用噬菌体展示技术来鉴定与与血清型8肺炎球菌荚膜多糖（PPS-8）反应的抗体结合的肽模拟表位。 这些肽将用作疫苗靶标，以确定它们是否在实验性肺炎球菌感染中引发保护性抗体反应。 SCID-hu 小鼠将用于研究抗体对肽的反应。 因此，可以研究人类抗体反应。 此外，生成的试剂可用于研究疫苗在不同患者组中引发的抗体的特异性。