NIH MACAQUE RESOURCES--DEFINITION OF HUMORAL IMMUNITY

NIH 猕猴资源——体液免疫的定义

• 批准号: 6394639
• 负责人: ROBERTA ATTANASIO
• 金额: $ 10.08万
• 依托单位: GEORGIA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6394639
• 关键词: Macaca mulatta; Macaca nemestrina; enzyme linked immunosorbent assay; genetic models; humoral immunity; immunoglobulin genes; laboratory rabbit; model design /development; northern blottings; southern blotting

The major objective of this proposal is to characterize further and define the humoral immune system of rhesus and pig-tailed macaques. These species are an invaluable and unique resource that are being used with increasing frequency for studies related to the pathogenesis, prophylaxis, diagnosis and therapy of a variety of human diseases. Rhesus and pig-tailed macaques are an important component of the NIH Regional Primate Research Center programs across the country and are essential to the HIV/SIV studies at the Centers and at numerous other NIH-funded institutions. Although cellular immune responses have been well characterized in macaques, limited information is available on their humoral immunity. The accurate definition of the humoral arm of the immune system, particularly and antibody repertoire, is imperative in order to validate the macaque model. To define the humoral immune system of these nonhuman primate species, we will analyze the macaque antibody repertoire at two different levels, genotypic phenotypic. Specifically, three specific aims are proposed to: 1) identify immunglobulin variable region genes and their products in rhesus and pig-tailed macaques; 2) identify immunoglobulin constant region genes and their products in rhesus and pig-tailed macaques; and 3) generate reagents specific for macaque immunoglobulin variable and constant regions and utilize these reagents to define antibody patterns in normal rhesus and pig-tailed macaques. Accomplishment of these specific aims will provide well-defined nonhuman primate models for understanding antibody-mediated protective and pathogenetic mechanisms and for the evaluation of the efficacy of novel immunointervention strategies.

该提案的主要目标是进一步描述和描述 定义恒河猴和猪尾猕猴的体液免疫系统。 这些物种是一种宝贵且独特的资源，正在被利用 随着与发病机制相关的研究越来越频繁， 预防、诊断和治疗多种人类疾病。 恒河猴和猪尾猕猴是猕猴的重要组成部分。 NIH 区域灵长类研究中心在全国范围内的项目 对于这些中心和众多机构的 HIV/SIV 研究至关重要 其他 NIH 资助的机构。 虽然细胞免疫反应 已在猕猴中得到很好的表征，信息有限 可以利用他们的体液免疫。 的准确定义 免疫系统的体液臂，特别是抗体 为了验证猕猴模型，剧目是必不可少的。 到 定义这些非人类灵长类动物的体液免疫系统， 我们将在两个不同水平上分析猕猴抗体库， 基因型表型。 具体来说，提出了三个具体目标 1) 鉴定免疫球蛋白可变区基因及其产物 恒河猴和猪尾猕猴； 2) 鉴定免疫球蛋白常数 恒河猴和猪尾猕猴的区域基因及其产物；和 3) 生成针对猕猴免疫球蛋白变量的特异性试剂和 恒定区并利用这些试剂来定义抗体模式 在正常恒河猴和猪尾猕猴中。 实现这些 具体目标将为以下领域提供明确定义的非人类灵长类动物模型 了解抗体介导的保护性和致病性 机制和新药功效评估 免疫干预策略。