METABOLIC CONTROL OF FEEDING BEHAVIOR

进食行为的代谢控制

• 批准号: 6329421
• 负责人: MARK FRIEDMAN
• 金额: $ 28.25万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6329421
• 关键词: adenosine triphosphate; antimetabolites; behavior prediction; bioenergetics; calcium flux; carbohydrate analog; laboratory rat; liver cells; liver metabolism; mannitol; nuclear magnetic resonance spectroscopy; nutrient intake activity; nutrition related tag; ouabain; psychobiology; sodium potassium exchanging ATPase

This is a request for five additional years support to carry out experiments involving metabolic subcellular changes in the liver and how they influence the onset of meals in rats. Prior evidence produced by the investigator suggests that when food intake is initiated after the administration of the metabolic inhibitor, 2, 5-AM, there are several correlates in hepatocytes that may well have a causal role. More specifically, the results of several types of experiments have converged to strongly implicate that the liver is the target for 2, 5-AMs or exigenicaction; and other experiments have indicated that a key factor (or at least close correlate) is a reduction in the amount of ATP in hepatocytes. Proposed experiments will take the analysis to a more reductionistic level by assessing whether bound or free cellular ATP is more correlated with eating and by then determining if a reduction in the key ATP pool elicit seating when induced by other means; will determine if other drugs thought to elicit eating via the liver (e.g., methyl palmoxirate) are associated with similar hepatocyte changes; will assess the hypothesis that the signal to eat is associated with functioning of the hepatocyte sodium pump; and will determine if changes in hepatocyte calcium pools are a means for generating a signal that could pass from hepatocytes to other cells and on the CNS.

这是额外五年支持的要求 涉及肝脏代谢亚细胞变化的实验以及如何 它们会影响大鼠的饭菜开始。先前的证据 研究人员建议，当摄入食物之后 代谢抑制剂2、5-AM的给药，有几个 可能具有因果关系的肝细胞中的相关性。更多的 具体而言，几种类型的实验的结果已收敛 强烈暗示肝脏是2、5 AM或 急性；其他实验表明，关键因素（或 至少密切相关）是ATP的量减少 肝细胞。提出的实验将使分析更多 通过评估绑定还是游离蜂窝ATP的降低水平为 与饮食相关，然后确定是否减少 当通过其他方式引起的关键ATP池池座位；将确定是否 其他被认为是通过肝脏引起进食的药物（例如甲基 2型甲状腺素与类似的肝细胞变化有关；将评估 饮食信号与功能有关的假设 肝细胞钠泵；并将确定肝细胞的变化 钙池是产生可能从中传递的信号的手段 肝细胞到其他细胞和中枢神经系统。