RYANODINE RECEPTOR REGULATION

兰尼碱受体调节

• 批准号: 6375085
• 负责人: JOHN L SUTKO
• 金额: $ 26.06万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6375085
• 关键词: Rana; calcium channel; chickens; cryoscience; laboratory rabbit; laboratory rat; muscle function; neuromuscular transmission; phosphopeptides; phosphorylation; protein isoforms; protein kinase; protein structure function; sarcoplasmic reticulum; species difference; western blottings

DESCRIPTION (Adapted from the Applicant's Abstract): The goal of the proposed studies is to test the hypothesis that activation of fast twitch skeletal muscles is regulated by altering the phosphorylation state of the ryanodine receptor (RyR) sarcoplasmic reticulum (SR) calcium release channels. In this project the investigator will use the back phosphorylation-fragmentation-derivatization (BFD) and fragmentation Western blot (frag-blot) methods to determine the in vivo phosphorylation stoichiometries of specific sites in the different RyR isoforms in vivo in nonactivated muscles, as well as during tetanic stimulation, and during moderate and severe fatigue. Rat extensor digitorum longus (EDL) and frog semitendinosus muscles will be compared o ascertain if phosphorylation of the RyRs differ in muscles containing different ratios of RyR isoforms. In addition, selected studies will be expanded to include rabbit and chicken muscles to test for interspecies variations between mammalian and nonmammalian species.

描述（改编自申请人的摘要）： 拟议的研究是为了检验快速激活的假设 通过改变磷酸化来调节抽搐骨骼肌 兰尼碱受体 (RyR) 肌浆网 (SR) 的状态 钙释放通道。在这个项目中，研究者将使用 反向磷酸化-断裂-衍生化（BFD）和 蛋白质印迹（frag-blot）方法测定体内片段化 不同 RyR 中特定位点的磷酸化化学计量 体内未激活的肌肉以及强直性痉挛期间的亚型 刺激以及中度和重度疲劳期间。 大鼠伸肌 将比较趾长肌 (EDL) 和青蛙半腱肌 o 确定 RyR 的磷酸化在包含以下物质的肌肉中是否不同： 不同比例的 RyR 同工型。 此外，选定的研究将 扩大到包括兔子和鸡的肌肉来测试 哺乳动物和非哺乳动物物种之间的种间变异。