MECHANISMS OF R RAS CELL ADHESION SIGNALING

R RAS 细胞粘附信号传导机制

• 批准号: 6376848
• 负责人: ANDREW M CHAN
• 金额: $ 11.45万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6376848
• 关键词: 3T3 cells; SDS polyacrylamide gel electrophoresis; binding proteins; biological signal transduction; cell adhesion; cell cycle; chimeric proteins; enzyme activity; gene expression; genetic regulation; integrins; mitogen activated protein kinase; mutant; nucleic acid sequence; plasmids; tissue /cell culture; transfection /expression vector; yeast two hybrid system

DESCRIPTION: (adapted from the investigator's abstract) This application aims to study the mechanisms involved in alterations in cell adhesion induced by ras related GTB binding protein R-ras. They hope to identify novel components leading to integrin activation and the subsequent search for their de-regulation in tumors. The specific aims are the following: 1) to identify which residues within R-ras code for sequences in cell adhesion. Chimeric mutants between R-ras and H-ras will be used. 2) The role of various effectors of R-ras mediating cell adhesion functions will be delineated. A panel of R-ras functional mutants with varying abilities to induce cell adhesion will be examined for their ability to interact with ras effectors. 3) Specific aim #3 will assess the role of R-ras in modulating cell adhesion by altering the downstream events mediated by ras such as the ras/raf MAP kinase pathway. In this specific aim the biologic consequences of crossed top between R-ras and ras will be explored with respect to regulating cell adhesion during cell division. In summary this proposal addresses some of the important and timely issues concerning the mechanisms by which ras related G proteins confer signaling specificity in controlling vital biologic functions such as cell adhesion.

描述：（改编自研究者的摘要）此应用程序 旨在研究细胞粘附改变的机制 由ras相关GTB结合蛋白R-ras诱导。 他们希望能够识别出 导致整合素激活和后续搜索的新成分 因其在肿瘤中的失调。 具体目标如下：1） 鉴定 R-ras 中的哪些残基编码细胞粘附序列。 将使用R-ras和H-ras之间的嵌合突变体。 2）作用 R-ras 介导细胞粘附功能的各种效应子将 划定。 一组具有不同能力的 R-ras 功能突变体 将检查诱导细胞粘附与 ras 相互作用的能力 效应器。 3) 具体目标#3将评估R-ras在调节中的作用 通过改变 ras 介导的下游事件（例如 ras/raf MAP 激酶途径。 在这个特定目标中，生物学后果 R-ras 和 ras 之间的交叉顶部将被探索 调节细胞分裂过程中的细胞粘附。 总结一下这个建议 解决了有关机制的一些重要和及时的问题 ras 相关 G 蛋白通过其赋予信号特异性来控制 重要的生物功能，例如细胞粘附。