CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6324782
• 负责人: EHUD LAVI
• 金额: $ 16.89万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6324782
• 关键词: biomedical facility; electron microscopy; immunocytochemistry; immunofluorescence technique; in situ hybridization; nervous system infection; neuropathology; neurotropic virus; polymerase chain reaction

The core facility will consolidate all the morphological services for the use of the various projects. These services include histological preparations such as tissue processing, embedding sectioning and staining. Following histological processing the core will provide evaluation and interpretation of histopathological changes in tissue sections. These include evaluation and quantification of demyelination during coronavirus infection in mice, evaluation of virus-induced cerebrovascular disease in murine leukemia virus infection in mice, and analysis of HSV receptor expression in human brain. Computerized morphometric analysis will be offered as necessary. Additional morphological services or assistance will be provided for studies which require immunohistochemistry, immunofluorescence, in situ hybridization, in situ-PCR, and electron microscopy. Thus the core facility services are essential to the success of the fundamental goal of the project.

核心设施将整合所有形态学服务 各种项目的使用。这些服务包括组织学 组织处理、包埋切片和染色等准备工作。 组织学处理后，核心将提供评估和 解释组织切片的组织病理学变化。这些 包括冠状病毒期间脱髓鞘的评估和量化 小鼠感染，病毒引起的脑血管疾病的评估 小鼠白血病病毒感染及HSV受体分析 在人脑中的表达。计算机形态测量分析将 根据需要提供。额外的形态学服务或协助将 为需要免疫组织化学的研究提供， 免疫荧光、原位杂交、原位 PCR 和电子 显微镜。因此，核心设施服务对于成功至关重要 项目的基本目标。