REGULATION OF CONNEXIN-43 GENE EXPRESSION IN THE UTERUS

子宫中 CONNEXIN-43 基因表达的调节

• 批准号: 6387805
• 负责人: RUDOLF K WERNER
• 金额: $ 26.95万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-06-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6387805
• 关键词: active sites; cell line; gap junctions; gel mobility shift assay; gene expression; gene targeting; genetic promoter element; genetic regulation; genetic translation; genetically modified animals; laboratory mouse; membrane channels; messenger RNA; molecular cloning; myometrium; progesterone; protein structure function; ribosomes; tissue /cell culture; transcription factor; uterus; yeast two hybrid system

DESCRIPTION: (Adapted from the Investigator's Abstract): The research plan is divided into two parts, regulation at the transcriptional level and regulation at the translational level. The first part of the project is the continuation of studies to characterize the proteins that are responsible for the induction of connexin43 in the myometrium. The functional characterization of one of these proteins, a novel transcription factor, Ini, that was discovered during the previous grant period, will be completed. Three additional transcription factors that were identified by Southwestern blot and DNA mobility shift analysis will be cloned and the function of these proteins in connexin43 gene regulation studied. The likely presence of an additional estrogen-responsive transcriptional activation site in the 5'-UTR of the connexin43 gene will also be investigated. The second part of this research is based on the recent discovery in this laboratory of an internal ribosome entry site (IRES) in the 5'-UTR of the connexin43 mRNA. The function of the IRES element will be studied in bicistroni vector constructs both in vivo and in vitro. In addition, the proteins that function in the activation of the connexin43 IRES will be identified by RNA mobility shift analysis and ribosome binding assays.

描述：（根据调查员的摘要改编）：研究计划是 分为两个部分，在转录级别和调节处进行调节 在翻译层面。该项目的第一部分是延续 研究负责诱导的蛋白质的研究 子宫骨值中的connexin43。一个功能表征之一 这些蛋白质是一种新颖的转录因子INI，在 上一个赠款期将完成。另外三个转录 通过西南印迹和DNA迁移率识别的因素 分析将被克隆，这些蛋白质在connexin43基因中的功能 调节研究了。可能存在额外的雌激素反应性 连接基因5'-UTR中的转录激活位点也将 被调查。这项研究的第二部分是基于最近的 在这个实验室中发现内部核糖体入口站点（IRES） 5'-utr connexin43 mRNA。将研究IRES元素的功能 在Bicistroni载体中，体内和体外构建。另外， 在connexin43 Ires激活中起作用的蛋白质将是 通过RNA迁移率分析和核糖体结合测定标识。