NMR AS A PROBE OF METAL ION BINDING SITES IN RNA

NMR 作为 RNA 金属离子结合位点的探针

• 批准号: 6484451
• 负责人: CHRISTOPHER V GRANT
• 金额: $ 1.78万
• 依托单位: WEIZMANN INSTITUTE OF SCIENCE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-26 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6484451
• 关键词: aminoacyl tRNA; binding sites; chemical chain length; chemical structure function; cobalt; computer simulation; crystallization; imaging /visualization /scanning; intermolecular interaction; ions; magnesium ion; metal complex; method development; model design /development; molecular site; nuclear magnetic resonance spectroscopy; nucleic acid structure; nucleotides; physical model; potassium ion; rubidium; sodium ion; structural biology

Metal ions such as magnesium, potassium and sodium are essential for the formation of tertiary structure in nucleic acids, and the enzymatic activity of catalytic RNAs. Magnesium and potassium ions are known to occupy specific binding sites in RNA that are formed by sophisticated macromolecular structure. Magnesium, potassium and sodium have isotopes of significant natural abundance that are half- integer spin quadrupole nuclei. Recent methodological and technological advances have allowed for solid state NMR studies of nuclei of this type. It is the goal of this study to apply solid state NMR as a structural probe of the interaction of these metal centers and the cobalt(III) hexammine ion with nucleotides and nucleic acids.

金属离子（例如镁，钾和钠）对于核酸中三级结构以及催化RNA的酶促活性至关重要。已知镁和钾离子在RNA中占据特定的结合位点，这些结合位点是由成熟的大分子结构形成的。镁，钾和钠的同位素具有明显的自然丰度，是半整数自旋四极核。最近的方法论和技术进步允许对这种类型的核进行固态NMR研究。这项研究的目的是将固态NMR作为这些金属中心与钴（III）抵可胺与核苷酸和核酸的相互作用的结构探针。