CORE--DNA SYNTHESIS FACILITY

核心——DNA合成设施

• 批准号: 6338767
• 负责人: THOMAS M. HARRIS
• 金额: $ 21.87万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338767
• 关键词: DNA; biomedical facility; chemical carcinogen; nucleic acid chemical synthesis; oligonucleotides

This laboratory will prepare and purify deoxyoligonucleotides for the various investigators. The facility will have two functions: preparation of unmodified oligomers derived from the four natural deoxynucleosides and preparation of carcinogen-modified oligomers or halogen-substituted oligomers for use in substitution reactions. Small quantities of the unmodified oligomers will be needed for genetics experiments; the facility will also prepare the larger quantities needed for NMR experiments. While the preparation of normal oligomers might be contracted out to commercial laboratories, the irreplaceable function of the laboratory will be to prepare structurally modified oligomers using novel reagents developed in Project 1. In some case only minute amounts of adducted oligomers will be required, i.e., for molecular biological experiments, but much of the proposed work will require relatively large quantities. The NMR experiments require milligram quantities since useful NOESY spectra generally require at least 2-4 mM concentrations in a volume of 0.4 mL; similarly X-ray diffraction studies require relatively large quantities for preparation of crystals.

该实验室将制备和纯化脱氧寡核苷酸，用于 各种调查员。 该设施将有两个功能：准备 源自四种天然脱氧核苷的未修饰寡聚物和 致癌物改性低聚物或卤素取代的制备 用于取代反应的低聚物。 少量的 遗传学实验需要未修饰的寡聚物；该设施 还将准备核磁共振实验所需的大量样品。 尽管 普通低聚物的制备可能会外包给商业公司 实验室的不可替代的功能是 使用开发的新型试剂制备结构修饰的低聚物 项目 1. 在某些情况下，只会产生微量的加合低聚物 需要，即分子生物学实验，但大部分 拟议的工作将需要相对较大的数量。 核磁共振 由于有用的 NOESY 光谱，实验需要毫克量 通常需要 0.4 mL 体积中至少 2-4 mM 浓度； 类似地，X 射线衍射研究需要相对大量的样品 晶体的制备。