Morphometric/immunocytochemical analysis of functional recovery in Parkinsonian

帕金森病功能恢复的形态测量/免疫细胞化学分析

• 批准号: 6353144
• 负责人: Don Marshall Gash
• 金额: $ 16.9万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6353144
• 关键词: Macaca mulatta; Parkinson's disease; autoradiography; brain mapping; central neural pathway /tract; disease /disorder model; dopamine; drug delivery systems; functional ability; histopathology; immunocytochemistry; in situ hybridization; nervous system regeneration; neuronal transport; neurotrophic factors

All three projects of this program focus on critically testing the central hypothesis that chronic infusions of GDNF promote the regeneration and functional recovery of the nigrostriatal dopamine system. Project 3 utilizes quantitative morphometric procedures, immunocytochemistry, in stu hybridization, immunoautoradiographic procedures and retrograde tract tracing to analyze the recovery of the nigrostriatal dopamine system in parkinsonian rhesus monkeys receiving GDNF. The effects of chronic infusions into two CNS sites are critically tested, the lateral ventricle and the putamen. The analysis includes determining the consequences of interrupting trophic factor for four months and then reinstating GDNF infusion for eight weeks. The results from these studies, combined with the neurochemical analyses in Project 1 and the behavioral and functional MRI measures of Project 2, should advance our understanding of the factors underlying successful regeneration in the parkinsonian brain. In addition, these studies significantly contribute to developing the methodology for chronically delivering other tropic factors and therapeutic compounds into the CNS.

该计划的所有三个项目都侧重于批判性测试中心假设，即GDNF的慢性输注促进了黑质纹状体多巴胺系统的再生和功能恢复。 项目3利用定量形态学程序，免疫细胞化学，在Stu杂交，免疫疗法过程中和逆行式追踪来分析帕金森氏鼠尾草猴子猴子麦芽粥样的骨膜纹状体多巴胺系统的恢复。 慢性输注对两个中枢神经系统部位的影响进行了严格的测试，侧心室和壳壳。 分析包括确定四个月中断营养因子的后果，然后恢复GDNF输注八周。 这些研究的结果与项目1的神经化学分析以及项目2的行为和功能性MRI度量相结合，应促进我们对帕金森氏症大脑成功再生的因素的理解。 此外，这些研究显着有助于开发慢性将其他热带因素和治疗化合物的方法发展到中枢神经系统中的方法。