CORE--RABBIT AND HUMAN BLADDER TISSUE

核心——兔和人膀胱组织

• 批准号: 6346143
• 负责人: Stephen Anthony Zderic
• 金额: $ 18.13万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6346143
• 关键词: animal genetic material tag; animal tissue; biomedical facility; human genetic material tag; human tissue; laboratory rabbit; smooth muscle; tissue resource /registry; urinary bladder; urinary bladder disorder; animal genetic material tag; animal tissue; biomedical facility; human genetic material tag; human tissue; laboratory rabbit; smooth muscle; tissue resource /registry; urinary bladder; urinary bladder disorder

The Bladder Tissue Core (Core B) will be used by all three projects in the Urology Research Center. The aim of the Bladder Tissue Core is to provide the program investigators with smooth muscle tissues from normal, obstructed and reversed rabbit bladders which have been characterized physiologically. It will also provide specimens from adult patients, with well-characterized symptoms of outlet obstruction, who are treated at the University of Pennsylvania Medical Center. In addition, protein extracts and RNA will be prepared from human and rabbit samples and distributed to various investigators for analyses. The Core will perform sham operations and surgery to produce partial outlet obstruction in rabbits, provide care for the animals postoperatively, obtain biopsies from bladders to monitor the degree of bladder smooth muscle hypertrophy and functional compensation, perform reversal surgery, sacrifice animals at appropriate time points, perform basic physiological characterization of the contractility of the bladder tissue, and distribute to the bladder tissues to the participating investigators. Physiological experiments will be performed with muscle strips to determine the ability of smooth muscle samples to produce force and with whole bladders to assess bladder emptying, since this property cannot be ascertained from isometric studies alone. Tissue samples will be either used immediately or frozen in liquid nitrogen for biochemical analysis or treatment with OCT for frozen sectioning for histology and immunohistochemistry and stored at -80 degrees Celsius for future use. All available information regarding the tissues at the time of distribution, or gathered subsequently from various projects in the Urology Research Center, will be logged onto a computer and made available to the investigators in the Center. Human detrusor smooth muscle tissues will be obtained from patients whose voiding dysfunction is clearly delineated based on urodynamic work-up and will be subdivided into those patients with low versus high post-void residual urine valves. Controls will be obtained from surgical specimens from non-cancerous regions of human bladder and from bladders of patient that have prostate cancers that did not produce an obstruction. Small muscle strips from human bladder tissue samples will be used for physiological studies to assess both the isometric force generating abilities as well as velocity of shortening. Additional human tissue will be frozen to allow for isolation of protein and RNA for correlation with physiologic studies. In studying a small number of well defined human bladder smooth muscle specimens, this core will help provide a crucial link between basic research findings and the clinical condition.

膀胱组织核心（核心B）将由所有三个项目都使用 泌尿外科研究中心。膀胱组织核心的目的是提供 该计划的调查员患有平滑肌组织，来自正常的肌肉组织 被妨碍和逆转了已表征的兔子膀胱 生理学上。它还将提供来自成年患者的标本， 出口阻塞的特征症状，在 宾夕法尼亚大学医学中心。另外，蛋白质提取物 RNA将从人和兔子样品中制备，并分布到 各种研究人员进行分析。 核心将进行假手术和手术以产生部分 兔子的出口阻塞，为动物提供护理 术后，从膀胱获取活检以监视 膀胱平滑肌肥大和功能补偿，执行 逆转手术，在适当的时间点牺牲动物，执行 膀胱收缩性的基本生理表征 组织，并分布到膀胱组织到参与 调查人员。生理实验将用肌肉进行 条带以确定平滑肌样品产生力的能力 并带有整个膀胱来评估膀胱排空，因为此属性 不能仅凭等距研究来确定。组织样品将是 立即使用或在液氮中冷冻作为生化 对OCT进行的分析或治疗，用于冷冻切片，以进行组织学和 免疫组织化学，储存在-80摄氏度以供将来使用。全部 有关分布时组织的可用信息， 或随后从泌尿外科研究的各个项目中收集 中心将登录到计算机上，并提供给 中心的调查人员。人类逼尿肌平滑肌组织将是 从缺乏功能障碍的患者中获得 基于尿动力的检查，并将细分为那些患者 与高接球后残留尿路相比低。控件将是 从人类非癌性区域的手术标本获得 膀胱和患有前列腺癌的患者膀胱 不产生阻塞。人膀​​胱组织的小肌肉条 样本将用于生理研究，以评估 等距产生能力以及缩短速度。 将批准其他人体组织以允许蛋白质分离 和与生理研究相关的RNA。在研究一个小 定义明确的人膀胱平滑肌标本，该核心 将有助于提供基础研究结果与 临床状况。