THERAPEUTIC EFFECT OF EMFS ON NEURAL PROCESSES/FUNCTIONS

EMFS 对神经过程/功能的治疗作用

• 批准号: 6312543
• 负责人: JEREMY B TUTTLE
• 金额: $ 16.68万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6312543
• 关键词: alternative medicine; apoptosis; biological signal transduction; brain injury; electromagnetic radiation; evaluation /testing; fibromyalgia; free radical scavengers; laboratory rat; magnetic field; nervous system regeneration; neural information processing; neurophysiology; stroke; synapses; tissue /cell culture

Magnetic devices and therapies are gaining rapid acceptance among some groups, even in the absence of reliable outcome data. This Center is focused upon the potential treatment with electromagnetic fields (EMFs) of fibromyalgia and the sequelae to CVA and closed head trauma. Despite considerable effort, it is still not clear how biological systems might be responding to therapeutically applied EMFs. The development and refinement of this potential new mode of therapy will be advanced considerably by a more explicit understanding of what basic biological processes are responsible for the desired therapeutic effects. This project will examine the effect of therapeutic magnetic fields upon specific neural processes and functions, in the effort to outline potential biosensors for EMFs in the nervous system. The tests will be conducted in vitro upon reasonable cellular reductionist models of events in the nervous system. In each case, EMF effects under basal and stressed conditions will be examined. Specific aims will measure magnetic field effects upon: l) Fiber regeneration by peripheral and central neurons. Primary neurons in culture will be used and the rate of regeneration of neurites measured. 2) Synapse formation and maturation. Cultures of peripheral neurons and muscle, as well as central neurons, will be examined for synapse formation and function at various times after isolation. 3) Calcium signaling via cytosolic transients in nerve and muscle cells. Fura-2 imaging will be used to examine EMF effects on IP3-mediated calcium signaling in primary cultures of muscle and human neuroblastoma cells. 4) Synthesis and release of neurotrophins. The production of NGF at the cellular level by muscle, neuroblastoma and glial cells will be examined. 5) Cell death and neuroprotective gene expression. EMF effects on induction of apoptosis and expression of free-radical scavengers and bcl-2 will be tested. Each of these test areas relates to a biological process involved in the response of the nervous system to injury or disorder and thus may be involved in the therapeutic efficacy of magnetic fields. The results of these largely exploratory experiments will outline likely basic biological processes capable of responding to EMF with a desirable therapeutic effect.

即使没有可靠的结果数据，磁性设备和疗法也正在迅速接受某些组。 该中心集中在纤维肌痛的电磁场（EMF）和后遗症和CVA和闭合头部创伤的潜在处理上。 尽管付出了巨大的努力，但仍不清楚生物系统如何应对治疗性EMF的反应。 通过更明确地了解哪些基本生物学过程负责所需的治疗作用，将大大提出这种潜在的新治疗方式的发展和完善。该项目将检查治疗磁场对特定神经过程和功能的影响，以概述神经系统中EMF的潜在生物传感器。 这些测试将在神经系统中合理的细胞还原主义模型中在体外进行体外进行。 在每种情况下，将检查基础和压力条件下的EMF效应。 具体目的将测量磁场对以下方面的影响：l）外周和中央神经元再生的纤维再生。将使用培养中的原发性神经元，并测量神经突的再生速率。 2）突触形成和成熟。 分离后的不同时间，将检查周围神经元和肌肉以及中央神经元的培养物以及突触形成和功能。 3）通过神经和肌肉细胞中的胞质瞬变通过钙信号传导。 Fura-2成像将用于检查对肌肉和人类神经母细胞瘤细胞原发性培养物IP3介导的钙信号传导的EMF影响。 4）神经营养蛋白的合成和释放。将检查肌肉，神经母细胞瘤和神经胶质细胞在细胞水平上的NGF产生。 5）细胞死亡和神经保护基因表达。 EMF对自由基清除剂和BCL-2的凋亡诱导的影响。 这些测试区域中的每一个都涉及神经系统对损伤或混乱的反应涉及的生物学过程，因此可能参与磁场的治疗功效。 这些很大程度上探索性实验的结果将概述能够以理想的治疗作用对EMF做出反应的基本生物学过程。