MOLECULAR MECHANISMS IN REOVIRUS TRANSCRIPTION & CAPPING

呼肠孤病毒转录的分子机制

基本信息

批准号：
6417605
负责人：
MAX L. NIBERT
金额：
$ 10.57万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-05-15 至 2002-03-09
项目状态：
已结题

项目摘要

The proposed studies will provide fundamental information about how the transcription and capping enzymes of mammalian reoviruses are organized within the subviral "core" particle. The general approach involves expression of the five core proteins (gamma1, gamma2, gamma3, mu2, and sigma2) for studies of their structure, function, and assembly into particles and more classical genetic and biochemical studies to associate the different activities in transcription, capping, and export of the viral messenger RNAs with specific core proteins and their domains. The long- term goal of this work is to characterize in a full molecular, structural, and mechanical sense how reovirus cores mediate these activities. The findings can be applied to understanding and controlling these processes in viruses and other pathogens, as well as in diseased and normal eukaryotic cells. The specific aims of the project are (1) to determine the structural positions and domain organizations of all five core proteins, (2) to investigate the RNA-dependent nucleoside triphosphatase activity in cores and to identify its role in transcription or capping, (3) to affiliate the four capping enzyme activities (RNA triphosphatase, RNA guanylyltransferase, RNA [guanine-7-N]-methyltransferase, and RNA [ribose- 2'-O]-methyltransferase) with specific proteins or protein regions in the core, and (4) to determine how the genomic dsRNA segments are organized and interact with proteins in the core.

拟议的研究将提供有关如何组织了哺乳动物埃默病毒的转录和上限酶内部“核心”粒子内。一般方法涉及五种核心蛋白的表达（gamma1，gamma2，gamma3，mu2和 Sigma2）用于研究其结构，功能和组装颗粒以及更经典的遗传和生化研究病毒的转录，封盖和导出的不同活动具有特定核心蛋白质及其域的Messenger RNA。长期这项工作的术语目标是表征完整的分子，结构，以及机械意义，旋孢病毒核如何介导这些活动。这调查结果可以应用于理解和控制这些过程病毒和其他病原体，以及患病和正常的真核细胞。该项目的具体目的是（1）确定所有五种核心蛋白质的结构位置和领域组织，（2）研究RNA依赖性核苷三磷酸酶活性核心并确定其在转录或封盖中的作用，（3）附属四种封封酶活性（RNA三磷酸酶，RNA 瓜尼林升转移酶，RNA [Guanine-7-N] - 甲基转移酶和RNA [核糖 - 2'-o] - 甲基转移酶）具有特定蛋白质或蛋白质区域核心，（4）确定基因组dsRNA段的组织方式，并与核心中的蛋白质相互作用。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

IRIS explorer software for radial-depth cueing reovirus particles and other macromolecular structures determined by cryoelectron microscopy and image reconstruction.

IRIS 浏览器软件，用于通过冷冻电子显微镜和图像重建确定径向深度提示呼肠孤病毒颗粒和其他大分子结构。

DOI：
10.1006/jsbi.1997.3902
发表时间：
1997
期刊：
Journal of structural biology
影响因子：
3
作者：
Spencer,SM;Sgro,JY;Dryden,KA;Baker,TS;Nibert,ML
通讯作者：
Nibert,ML

Structure of mammalian orthoreovirus particles.

哺乳动物正呼肠孤病毒颗粒的结构。

DOI：
10.1007/978-3-642-72092-5_1
发表时间：
1998
期刊：
Current topics in microbiology and immunology
影响因子：
0
作者：
Nibert,ML
通讯作者：
Nibert,ML

Thermostability of reovirus disassembly intermediates (ISVPs) correlates with genetic, biochemical, and thermodynamic properties of major surface protein mu1.

呼肠孤病毒分解中间体 (ISVP) 的热稳定性与主要表面蛋白 mu1 的遗传、生化和热力学特性相关。

DOI：
10.1128/jvi.76.3.1051-1061.2002
发表时间：
2002
期刊：
Journal of virology
影响因子：
5.4
作者：
Middleton,JasonK;Severson,TonyaF;Chandran,Kartik;Gillian,AnneLynn;Yin,John;Nibert,MaxL
通讯作者：
Nibert,MaxL

Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein.