MUCOSAL IMMUNE RESPONSES DURING CRYPTOSPORIDIOSIS

隐孢子虫病期间的粘膜免疫反应

• 批准号: 6170302
• 负责人: Carol R. Wyatt
• 金额: $ 1.02万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6170302
• 关键词: Cryptosporidium; T lymphocyte; antigen antibody reaction; clone cells; cow; cryptosporidiosis; enzyme linked immunosorbent assay; epitope mapping; feces analysis; flow cytometry; host organism interaction; immunocytochemistry; immunoprecipitation; light microscopy; mucosal immunity; pathologic process; polymerase chain reaction; protozoal antigen; western blottings

Cryptosporidium parvum is a coccidian parasite that infects intestinal and extraintestinal mucosal epithelial cells in humans, cattle and other mammals. C. Parvum causes self-limiting gastroenteric disease in immune competent hosts, but becomes persistent and life-threatening in immunocompromised individuals. Several recent outbreaks of cryptosporidiosis have occurred, primarily in association with water supplies. There is no effective antimicrobial drug available to treat infection, and no vaccine to prevent disease. Development of these remedies will require understanding what is likely a complex series of immune responses to c. Parvum. It will be especially important to distinguish between protective immunity (leading to termination of infection) and an immune response that might contribute to disease. My long-term goals are: 1) identify antigenic epitopes of C. parvum that elicit protective immunity, and 2) determine whether specific epitopes induce immune responses that contribute to clinical disease in susceptible hosts. The research proposed in this application constitutes an important first step toward these goals, because it involves identifying the sequence of immune responses that occur in intestinal mocosa during the course of disease in a susceptible, immune competent host. My hypothesis is that the immune response induced by C. parvum early in infection differs from that which occurs in conjunction with termination of infection. Specific Aims 1. Document the ileal mucosal immune responses that occur during the course of infection. 2. Characterize in vitro responses to C. parvum antigens of lymphocyte collected from local, regional, and systemic immune compartments. 3. Develop T cell clones from lymphocytes taken during the course of infection, and characterize their responses to stimulation with fractionated C. parvum parasites.

隐孢子虫帕尔瓦姆（Cryptosporidium parvum 以及人类，牛和其他 哺乳动物。 C. Parvum在 免疫胜任的宿主，但变得持久而威胁生命 在免疫功能低下的个体中。 最近的几次爆发 发生隐孢子虫病发生，主要与水有关 补给品。 没有可用的有效抗菌药物可以治疗 感染，没有预防疾病的疫苗。 这些的发展 补救措施将需要了解什么可能是一系列复杂的 免疫反应c。 parvum。 这将特别重要 区分保护性免疫（导致终止 感染）和可能导致疾病的免疫反应。 我的长期目标是：1）确定C. parvum的抗原表位 这种引起保护性免疫，2）确定是否具体 表位诱导免疫反应，导致临床疾病 易感宿主。 本申请中提出的研究构成 朝着这些目标迈出的重要第一步，因为它涉及 识别肠中发生的免疫反应的序列 在易感性免疫的疾病过程中，Mocosa 主管主持人。 我的假设是免疫反应诱导 在感染早期的C. parvum与发生在感染的情况下不同 与终止感染的结合。 具体目标 1。记录在此期间发生的回肠粘膜免疫反应 感染过程。 2。表征对C. parvum抗原的体外反应 从局部，区域和全身免疫收集的淋巴细胞 车厢。 3。从淋巴细胞中开发T细胞克隆。 感染，并表征他们对刺激的反应 分离的C. parvum寄生虫。