MULTIUSER AFFYMETRIX GENE CHIP SYSTEM

多用户 AFFYMETRIX 基因芯片系统

• 批准号: 6291412
• 负责人: PHILIP W. LANDFIELD
• 金额: $ 21.45万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291412
• 关键词: biomedical equipment; biomedical equipment purchase; microarray technology; nucleic acid sequence

DESCRIPTION: (Investigator?s Abstract): This is a proposal to establish a comprehensive DNA Microarray Analysis and Bioinformatics System at the University of Kentucky. DNA microarray methods clearly will revolutionize future studies of gene expression. Numerous NIH-supported projects here already focus on gene expression and regulation approaches, in many cases with transgenic animals. Therefore, the ability to incorporate large-scale gene array analyses would vastly accelerate progress, refine interpretation, and drive the discovery of novel expression-function correlations in these projects. At present, expression analyses are performed here with slow, labor-intensive and/or limited methods (e.g., PCR, small chips, Northerns, etc.). Gene array capability would also be of particular benefit at Kentucky because many of the major users? projects emphasize quantitative comparisons and detection of subtle differences in gene expression (e.g., with aging, exposure to growth factors, hormones or toxicants, brain injury, circadian rhythms, carcinogenesis, signaling cascades, etc). The integrated system requested here (Affymetrix), is the only fully comprehensive system (chips, hybridization oven, fluidics station, laser analyzer, bioinformatics package) presently available, and several of our faculty are experienced in its use. This system also appears to be the most sensitive, accurate and standardized, characteristics that would be a key benefit to the quantitatively oriented projects at the University of Kentucky. In addition, several of the projects at Kentucky are developing and refining novel methods for expression studies in functionally characterized single orhomogenous cells in order to more accurately study correlations between expression and function. A component of this work involves the representative amplification of sufficient quantities of mRNA to allow expression profiling in single cells. Development of these approaches would be greatly facilitated by gene array profiles that could provide detailed information on how representative and reproducible the various amplification procedure are. Thus, the proposed system would not only specifically enhance progress in multiple NIH-funded projects at the University of Kentucky, but would facilitate the development of new methods that may well benefit investigators at many other institutions as well.

描述：（研究者的摘要）：这是建立一个的建议 全面的DNA微阵列分析和生物信息学系统 肯塔基大学。 DNA微阵列方法显然会革新 基因表达的未来研究。这里已经有许多由NIH支持的项目 在许多情况下，专注于基因表达和调节方法 转基因动物。因此，合并大型基因的能力 阵列分析将极大地加速进度，完善的解释和 驱动这些新型表达功能相关性的发现 项目。目前，在这里以缓慢的方式进行表达分析 劳动密集型和/或有限的方法（例如PCR，小芯片，北部， ETC。）。肯塔基州的基因阵列能力也将特别有益 因为许多主要用户？项目强调定量比较 以及检测基因表达微妙差异（例如，随着衰老， 暴露于生长因子，激素或有毒物质，脑损伤，昼夜节律 节奏，癌变，信号级联反应等）。集成系统 在这里要求（Affymetrix），是唯一全面的系统（芯片， 杂交烤箱，流体站，激光分析仪，生物信息学包装） 目前可用，我们的一些教师在使用方面经验丰富。 该系统似乎也是最敏感，准确和标准化的， 特征将是定量取向的关键优势 肯塔基大学的项目。此外，在 肯塔基州正在开发和完善新的方法来表达研究 在功能上表征了单个神性细胞，以便更多 准确研究表达与功能之间的相关性。一个组成部分 这项工作涉及足够数量的代表性放大 mRNA允许在单细胞中进行表达分析。这些的发展 基因阵列轮廓可以极大地促进方法 提供有关各种代表性和可再现方式的详细信息 放大程序是。因此，拟议的系统不仅将 特别提高了大学多个NIH资助项目的进展 肯塔基州的著作，但将促进开发可能的新方法 在许多其他机构中也受益。