BRUKER ELEXSYS EPR SPECTROMETER

布鲁克 ELEXSYS EPR 光谱仪

• 批准号: 6291972
• 负责人: Jimmy Feix
• 金额: $ 42.1万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-15 至 2002-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6291972
• 关键词: biomedical equipment purchase; electron spin resonance spectroscopy

The purpose of this proposal is to provide access to a reliable, state-of- the-art X-band CW EPR spectrometer with high sensitivity and time resolution at the Biophysics Research Institute of the Medical College of Wisconsin. Each of these projects employs EPR as the primary analytical tool. The requested instrument will allow the faculty to pursue their NIH-funded projects with a rigor not otherwise possible. Specifically, the projects that will benefit include (1) characterization of ligand-induced conformational changes in the ferric enterobactin receptor as studied by site-directed spin labeling, (2) studies of oxidation/nitration by nitric oxide, (3) reactions between nitric oxide, peroxynitrite, and thiols, (4) analysis of free radical mechanisms of LDL oxidation, (S) examination of endothelial nitric oxide synthase and the radical mechanism of antitumor anthracyclines, (6) EPR studies of the antitumor agent bleomycin and its complexes with DNA, (7) spin label oximetry investigations, (8) studies of the molecular basis of lens transparency, and (9) site-directed spin labeling studies of small heat- shock proteins. Each of these projects has a clear need for increased access to a research-level EPR spectrometer. This proposal seeks funding for an instrument with high sensitivity, accurate temperature control in the range of 4 - 400 K, well-developed and adaptable software, and time resolution on the order of a few milliseconds. EPR is a fundamental technique in studies of protein and metalloprotein structure and in elucidating free radical intermediates in biochemical and pathological processes. Investment in the requested spectrometer will substantially strengthen NIH-supported research.

该提案的目的是在威斯康星州医学院生物物理学研究所提供可靠的，最先进的X波段CW EPR光谱仪，具有高灵敏度和时间分辨率。 这些项目中的每一个都采用EPR作为主要的分析工具。所请求的工具将使教师以其他方式严格地从事其NIH资助的项目。具体而言，将受益的项目包括（1）通过位置定向的自旋标记所研究的铁肠乳素受体中配体诱导的构象变化的表征，（2）通过一氧化氮对氧化/硝化作用的研究，（3）硝酸氧化物，氧化氧化氧化物，过氧基质和thiols（4）的氧化氧化物反应（3）（4）的氧化（4）分析（4）透射率的分析（4）透射率（4）透明度的分析（4）的氧化作用。 endothelial nitric oxide synthase and the radical mechanism of antitumor anthracyclines, (6) EPR studies of the antitumor agent bleomycin and its complexes with DNA, (7) spin label oximetry investigations, (8) studies of the molecular basis of lens transparency, and (9) site-directed spin labeling studies of small heat- shock proteins.这些项目中的每一个都清楚地需要增加对研究级EPR光谱仪的访问。该提案寻求具有高灵敏度，精确温度控制范围的仪器的资金，在4-400 K范围内，发达且适应性的软件以及几毫秒级的时间分辨率。 EPR是蛋白质和金属蛋白结构研究以及阐明生化和病理过程中自由基中间体的基本技术。对要求的光谱仪的投资将大大加强NIH支持的研究。