SEX-RELATED OPIATE AND AUTONOMIC MECHANISMS IN TMD PAIN

TMD 疼痛中与性相关的阿片类药物和自主机制

• 批准号: 6379859
• 负责人: DAVID A BEREITER
• 金额: $ 29.74万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379859
• 关键词: afferent nerve; analgesia; autonomic nervous system; brain stem; disease /disorder model; dorsal horn; electrophysiology; gender difference; immunocytochemistry; laboratory rat; microdialysis; morphine; neurons; neurotransmitter transport; oral facial pain; spinal trigeminal nucleus; temporomandibular joint syndrome

Temporomandibular disorders (TMD) to a family of conditions that cause pain of the temporomandibular joint (TMJ) region and surrounding deep craniofacial muscles. Although the etiology of TMD's may vary, this facial pain condition is commonly accompanied by long-term changes in sensory, motor reflex and autonomic function suggesting a central dysfunction. TMD is notable for its higher prevalence in women than in men and for its recurrent nature. Despite evidence for an underlying central neural dysfunction, little is known of the brain mechanisms necessary for TMD pain. The central hypothesis of this proposal is that sex-related differences in TMD pain reside, at least in part, at the level of second-order neurons within the spinal trigeminal nucleus and upper cervical dorsal horn. Since the neurons that supply the tissues of the TMJ region terminate in the trigeminal brainstem complex and upper cervical spinal cord, the activity of second-order neurons within these regions must assume a critical importance in determining the various aspects of nociception (e.g., sensory-discrimination, autonomic reflexes, recruitment of descending controls). The proposed studies use an animal model to selectively activate small diameter afferent nerves and converging methodologies (c-fos) immunocytochemistry, in vivo microdialysis, single neuron electrophysiology) to examine the properties of second-order neurons that receive noxious sensory input from the TMJ region in anesthetized male and female rats. The Specific Aims examine the effects of the well-known activators of endogenous pain control systems, morphine and vagal afferent nerve activity, to assess the properties of TMJ-responsive neurons in male and female rats. The long-term goal of this research is to better understand the influences of brain-endocrine-autonomic interactions in orofacial pain processing. The shorter term goal is to define the involvement of opiate and vagal afferent nerve mechanisms at the level of the trigeminal brainstem complex that may have analgesic effects after inflammation of the TMJ region.

颞下颌关节紊乱病 (TMD) 是导致 颞下颌关节 (TMJ) 区域及周围深部疼痛 颅面肌。 尽管 TMD 的病因可能有所不同，但 面部疼痛状况通常伴随着长期的变化 感觉、运动反射和自主神经功能表明中枢 功能障碍。 值得注意的是，TMD 在女性中的患病率高于男性 男人及其反复出现的性质。 尽管有证据表明存在潜在的 中枢神经功能障碍，对大脑机制知之甚少 TMD 疼痛所必需的。 该提案的中心假设是 TMD 疼痛的性别相关差异至少部分在于 三叉神经脊髓核内二级神经元的水平 上颈背角。 由于供应组织的神经元 TMJ 区域终止于三叉神经脑干复合体 上颈脊髓，内二级神经元的活动 这些地区在确定 伤害感受的各个方面（例如，感觉辨别、自主神经 反射，招募下降控制）。 拟议的研究使用 选择性激活小直径传入神经的动物模型 和聚合方法 (c-fos) 体内免疫细胞化学 微透析、单神经元电生理学）来检查 接收有害感觉输入的二阶神经元的特性 来自麻醉的雄性和雌性大鼠的颞下颌关节区域。 具体 目的检查众所周知的内源性疼痛激活剂的作用 控制系统、吗啡和迷走神经传入神经活动，以评估 雄性和雌性大鼠颞下颌关节反应神经元的特性。这 这项研究的长期目标是更好地了解影响 口面部疼痛处理中大脑-内分泌-自主神经相互作用的研究。 短期目标是定义阿片类药物和迷走神经的参与 三叉神经脑干水平的传入神经机制 颞下颌关节炎症后可能具有镇痛作用的复合物 地区。