LONG TERM RECORDING FROM AFFERENT TASTE FIBERS

味觉传入纤维的长期记录

• 批准号: 6379509
• 负责人: Robert M Bradley
• 金额: $ 23.79万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-29 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6379509
• 关键词: afferent nerve; bioengineering /biomedical engineering; biomedical equipment development; cell cycle; chorda tympani; electronic recording system; implant; laboratory rat; longitudinal animal study; microelectrodes; miniature biomedical equipment; nervous system regeneration; neurofilament; single cell analysis; taste buds; telemetry

Development of an implantable, multielectrode array has made it possible to record chronically, over periods of several weeks, from sensory fibers of taste nerves that innervate the tongue. In the current proposal, the electrode array will be used to systematically test hypotheses about neural discharge properties of peripheral taste fibers over long time frames that encompass numerous cycles of taste bud cell turnover, and synapse remodeling. In addition, the multielectrode recording device will be progressively refined to improve and expand its range of capabilities. The rat chorda tympani nerve will be dissected and implanted with the multielectrode array through which taste nerve fibers regenerate. Subsequently, recordings will be made from the same single fiber, or sets of fibers, for weeks. In Specific Aim 1, chronic recordings from single taste fibers of the chorda tympani nerve will be used to test the hypotheses that response characteristics of taste fibers, and receptive field properties, remain stable during cycles of taste bud cell turnover and synapse remodeling. In Specific Aim 2, simultaneous recordings from several individual fibers will be studied over weeks to test the hypothesis that there is a unique pattern of fiber activity for separate chemical qualities, and that the pattern remains stable over time. In Specific Aim 3, an implantable telemetry system will be used with the multielectrode array to record from chorda tympani fibers in awake rats and investigate the pattern of neural activity during feeding and drinking. The implantable, multielectrode recording array and its further refinement make possible tests of hypotheses about basic taste neurobiology that could not be resolved with previous neurophysiological techniques. Also, studies of taste fiber responses in behaving animals become feasible with the implantable device. Furthermore, continued refinement of, and experiments with, the implantable electrode array can lead to potential applications as a neural prosthesis in regeneration and restoration of sensory and motor function after trauma or disease.

从支配舌头的感觉神经的感觉纤维中，在几周的时间内，可以长期记录植入的多电极阵列的发展。在当前的提案中，电极阵列将用于系统地测试有关长时间框架外周味纤维神经放电特性的假设，该特性涵盖了许多味道芽细胞更新和突触重塑的周期。 此外，将逐步完善多电极记录设备，以改善和扩展其功能范围。 将解剖大鼠鼓膜神经并用多电极阵列植入并植入味道神经纤维再生的多电极阵列。 随后，录音将用相同的单个光纤或一组纤维制成数周。 在特定的目标1中，将使用Chorda Tympani神经的单个味觉纤维的慢性记录来测试味道纤维响应特征的假设，并且在味道芽细胞周转和突触重塑过程中保持稳定。 在特定的目标2中，将在数周内研究来自几个单个纤维的同时记录，以测试以下假设：单独的化学品质具有独特的纤维活性模式，并且该模式随着时间的推移仍然稳定。 在特定的目标3中，将与多电极阵列一起使用可植入的遥测系统，以记录清醒大鼠中的鼓膜纤维，并研究进食和饮用过程中神经活动的模式。 可植入的多电极记录阵列及其进一步的完善，可以对基本味道神经生物学的假设进行测试，这些假设无法通过以前的神经生理技术解决。 此外，对行为动物的味觉纤维反应的研究与植入式装置有关。此外，对植入电极阵列的持续细化和实验可以导致潜在的应用作为神经假体在创伤或疾病后感觉和运动功能的再生和恢复。