PHYTOREMEDIATION OF TOXIC WASTE SITES

有毒废物场地的植物修复

• 批准号: 6271040
• 负责人: MILTON P GORDON
• 金额: $ 23.32万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271040
• 关键词: Agrobacterium; angiosperms; biomarker; bioreactors; biotransformation; detoxification; environmental contamination; environmental toxicology; field study; genetic promoter element; genetic strain; genetically modified plants; industrial waste; soil pollution; toxin metabolism; trichloroethylene; water pollution

We plan to determine the ability of plants to remediate toxic waste sites. In prototype experiments, we will focus on the ability of polar trees to detoxify trichloroethylene (TCE). Poplars are able to grow in the presence of high levels of TCE and to transpire and metabolize the compounds. TCE is converted to trichloroethanol, trichloroacetic acid, and two polar compounds whose structures will be determined. We will determine which clones metabolize the largest proportion of TCE absorbed by the plants. The extent of metabolism will be augmented by introducing genes which are known to metabolize TCE. Laboratory studies of the uptake and metabolism of TCE will use gas-tight glass apparatuses with separate chambers for roots and crowns in order to determine the fate of 100: of the material administered. The best clones will be studied in greenhouse-scale experiments to determine their ability to absorb and detoxify TCE. Finally, field studies will be conducted in collaboration with the Occidental Chemical Corporation at a site at the Port of Tacoma. These field studies will take place for 2 to 4 years, will be heavily instrumented, will determine the ability of the plants to take up and detoxify TCE, and will measure the extent of transpiration of the compound. In conjunction with these studies, we will collaborate with Dr. Leah Bauer, USDA, East Lansing, MI, to determine the effect of the ingestion of chicks to determine whether there is any effect on these animals. We will also investigate whether we can use the ability of plants to metabolize. TCE to chlorinated products as an environmental biomarker of the extent of below-ground TCE contamination. Plant tissue from a range of sites will be analyzed for TCE metabolites and compared to known levels of TCE contamination. The use of plants to remediate pollution meets the mandate of the NIEHS to attenuate adverse effects of toxic materials on human health. This approach serves as a "green solution" to pollution abatement and is expected to meet with public approval.

我们计划确定工厂修复有毒废物场的能力。 在原型实验中，我们将重点关注极地树木的能力 解毒三氯乙烯（TCE）。 杨树能够在存在下生长 高水平的 TCE 并蒸发和代谢化合物。 三氯乙烯 转化为三氯乙醇、三氯乙酸和两种极性物质 其结构将被确定的化合物。 我们将确定哪个 无性系代谢植物吸收的最大比例的TCE。 通过引入以下基因可以增强新陈代谢的程度： 已知可代谢 TCE。 摄取和代谢的实验室研究 TCE 将使用带有独立室的气密玻璃装置 根和冠以确定100：材料的命运 管理。 最好的克隆将在温室规模上进行研究 实验以确定它们吸收和解毒三氯乙烯的能力。 最后，将与以下机构合作进行实地研究 西方化学公司在塔科马港的一个地点。 这些 实地研究将持续 2 至 4 年， 仪器化，将确定植物吸收和 对 TCE 进行解毒，并测量化合物的蒸腾程度。 结合这些研究，我们将与 Leah Bauer 博士合作， 美国农业部，密歇根州东兰辛，确定摄入雏鸡的影响 以确定是否对这些动物有任何影响。 我们还将研究是否可以利用植物的能力 代谢。 TCE 将氯化产品作为环境生物标志物 地下TCE污染的程度。 来自一系列的植物组织 将分析位点的 TCE 代谢物并与已知水平进行比较 三氯乙烯污染。 使用植物修复污染符合 NIEHS 的任务 减轻有毒物质对人体健康的不利影响。 这 该方法是减少污染的“绿色解决方案”， 预计将得到公众的认可。