CAPRINE ARTHRITIS ENCEPHALITIS VIRUS INFECT: IMMUNITY, GENOME: GOAT AIDS MODEL

山羊关节炎脑炎病毒感染：免疫力，基因组：山羊艾滋病模型

• 批准号: 6475067
• 负责人: EMMANUEL G MDURVWA
• 金额: $ 3.95万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6475067
• 关键词: AIDS; arthritis; biotechnology; communicable diseases; connective tissue; hematology; immunology; inborn metabolism disorder; inflammation; lymphatic system; minority institution research support; model design /development; nervous system; virus

Caprine arthritis encephalitis virus (CAEV) belongs to the lentivirus subfamily of retroviruses. It integrates into the host cell genome and induces a persistent infection of domestic goats. CAEV infection causes encephalomyelitis in young goats, and mastitis, chronic progressive arthritis and synovitis in adult goats. The arthritis which results is similar in pathology to rheumatoid arthritis (RA) in humans. There is recent evidence that a novel CAEV variant occurs in humans and generates immune cross-reactivity to human immunodeficiency virus- I (IUV- 1). It is evident that CAEV is important not only as a pathogen in goats but as a vehicle for studying human diseases. We will use CAEV-induced disease 'in goats as a model to study the pathogenetic mechanisms of lentiviruses and also to investigate further some of the mechanisms that lead to arthritis. The obiective of this proposed project is to study molecular mechanisms of CAEV pathogenesis. This is important in the light of the possible use of CAEV-1ike lentivuiuses as -prophylactic agents against human viruses like EDIV-1. Specifically we propose to: i) investigate virus-host cell interactions by identifying and characterizing cell surface receptor(s) and defining the role of viral gp 13 5 and i do tha are essential for interaction with the cellular receptors; and ii) investigate some mechanisms that may be involved in the initiation and progression of arthritis by determining the role of various cytokines and chemokines, oxygen radicals, intracellular calcium fluctuations, circulating immune complexes, rheumatoid factor and also by identifying genes that are differentially transcribed during the infection. These results will provide a better understanding of a disease condition that is very similar to RA and in which the etiology is known.

Caprine关节炎病毒（CAEV）属于 逆转录病毒的慢病毒亚科。 它集成到主机 细胞基因组并诱导家山羊的持续感染。 CAEV感染会导致年轻山羊和乳腺炎的脑脊髓炎， 成年山羊的慢性进行性关节炎和滑膜炎。 这 关节炎在病理学上与类风湿类似的关节炎 人类关节炎（RA）。 最近有证据表明一个新颖的CAEV 变体发生在人类中，并产生免疫交叉反应性 人类免疫缺陷病毒I（IUV-1）。 显然Caev是 重要的不仅是山羊病原体的重要 研究人类疾病。 我们将在山羊中使用CAEV诱导的疾病 作为研究慢病毒和 还要研究进一步导致的一些机制 关节炎。 这个拟议项目的概念是研究 CAEV发病机理的分子机制。 这在 可能使用Caev -1ike慢烟作为prophylactic的光 反对EDIV-1等人类病毒的代理。 具体来说，我们建议： i）通过鉴定和 表征细胞表面受体并定义病毒的作用 gp 13 5，我做到与细胞相互作用至关重要 受体； ii）研究可能涉及的一些机制 通过确定关节炎的启动和进展 各种细胞因子和趋化因子，氧自由基，细胞内 钙波动，循环免疫复合物，类风湿因子 并通过识别差异转录的基因 在感染期间。 这些结果将提供更好的 了解与RA非常相似的疾病状况 病因是已知的。