Core--Instrumental and analytical measurements

核心——仪器和分析测量

• 批准号: 6315346
• 负责人: Ulrich Hopfer
• 金额: $ 25.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-15 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6315346
• 关键词: biomedical facility; confocal scanning microscopy; electroporation; fluorescence microscopy; intracellular transport; microscopy; protein localization

The Instrumentation Core will provide all projects and principle investigators of this Program Project Grant with: a) confocal and conventional light microscopy and image analyses of fixed samples for localization and colocalization experiments, as well as quantification of morphological changes. b) ratiometric fluorescence microscopy of live cells for dynamic measurements of intracellular ion concentration of membrane potential changes. c) microinjection of proteins and nucleic acids to modulate intracellular message or signaling systems. d) injection of proteins into cells by electroporation to effect modulation of intracellular signaling system in biochemical studies. e) routine resistance and potential measurements of epithelial monolayers as quality control. f) quantitative analyses of protein and nucleic aid gels and immuno-blots. The staff of the Core will carry out measurements and provide assistance and counseling in planning of measurements and data analyses.

仪器核心将为该计划项目拨款的所有项目和主要研究人员提供：a）用于定位和共定位实验的固定样品的共焦和传统光学显微镜和图像分析，以及形态变化的量化。 b)活细胞比率荧光显微镜，用于动态测量细胞内离子浓度的膜电位变化。 c) 显微注射蛋白质和核酸以调节细胞内信息或信号系统。 d) 在生化研究中通过电穿孔将蛋白质注射到细胞中以影响细胞内信号系统的调节。 e) 上皮单层的常规电阻和电位测量作为质量控制。 f) 蛋白质和核酸凝胶和免疫印迹的定量分析。核心工作人员将进行测量，并在测量规划和数据分析方面提供帮助和咨询。