CELL ISOLATION AND UPKEEP

细胞隔离和维护

• 批准号: 6300498
• 负责人: N S LITOFSKY
• 金额: $ 17.2万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300498
• 关键词: biomedical facility; cell line; embryo /fetus tissue /cell culture; embryonic stem cell; immunocytochemistry; laboratory mouse; neoplastic cell; neurons; polymerase chain reaction; tissue /cell culture

The Neural Stem Cell and Neuroepithelial Tumor Isolation and Upkeep Core will coordinate maintenance of neural stem cells and neoplastic cell lines. It will also coordinate distribution of these cells to various individual projects. This core will maintain a library of frozen neurosurgical specimens for use in the various projects. Fetal, post- natal, and adult stem cells will be harvested in the core. The core will use cell culture techniques to provide these services. Because each of the projects requires the use of these cells, the core will ensure quality control of the primary research substrates of the program project. Quality control will be ensured by 1) standard cell culture techniques maintaining low passage number, 2) immunohistochemical staining, 3) RT-PCR banding patterns, and 4) timed pregnancies of the animals from which the fetal cells are harvested.

神经干细胞和神经上皮肿瘤的隔离和保养核心 将协调神经干细胞和肿瘤细胞的维护 线。 它还将协调这些细胞的分布到各种 个别项目。 该核心将保持冷冻的库 用于各种项目的神经外科标本。 胎儿，后 核心细胞和成年干细胞将在核心中收获。 核心 将使用细胞培养技术提供这些服务。 因为 每个项目都需要使用这些单元格，核心将 确保该计划的主要研究基材的质量控制 项目。 质量控制将通过1）标准细胞培养 维持低通道的技术，2）免疫组织化学 染色3）RT-PCR带模式和4）定时怀孕 收获胎儿细胞的动物。