NEURAL AND GI SUBSTRATES IN BOMBESIN SATIETY

蟾蜍素饱足感的神经和胃肠道底物

• 批准号: 6380792
• 负责人: ELLEN E LADENHEIM
• 金额: $ 25.25万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6380792
• 关键词: anorexic agent; antisense nucleic acid; appetite regulatory center; bioenergetics; bombesin; bombesin like peptide; brain mapping; caudate nucleus; gastrin releasing peptide; hormone regulation /control mechanism; immunocytochemistry; implant; laboratory rat; neural information processing; neuropeptide receptor; nutrient intake activity; nutrition related tag; oligonucleotides; satiations; stereotaxic techniques

DESCRIPTION (adapted from applicant's abstract): The overall aim of the research proposed in this application is to identify the role of central and peripheral mammalian bombesin-like (BN-like) peptides in the control of food intake. Two mammalian BN-like peptides have been identified: gastrin releasing peptide (GRP) and neuromedin B (NMB). These peptides and their corresponding receptors are differentially distributed in the gastrointestinal tract and central and peripheral nervous systems. BN-like peptides inhibit food intake following peripheral or central administration and these inhibitions appear to be mimicking actions of the endogenous peptides since both GRP and NMB receptor antagonist administration increases food intake. The proposed experiments examine the specific hypotheses that: 1) the receptor subtype mediation of peripheral and central BN-like peptides, differs; 2) the mediation of the satiety actions of peripheral BN-like peptides depends upon activation of a peripheral afferent pathway that results in a caudal hindbrain release of GRP; and 3) the central pathways mediating the satiety actions of central and peripheral BN-like peptides differ. The experiments addressing these hypotheses will employ specific antagonists and antisense oligonucleotides, c-fos immunohistochemical mapping, peripheral and central lesions and microstructural behavioral analyses. Finally, the investigators propose experiments to identify interactions between mammalian BN-like peptides and other peptides with anorexigenic actions to better understand the overall contribution of BN-like peptides in the controls of food intake. Together, the results of these experiments will significantly enhance our understanding of the actions of brain/gut peptides in food intake and body weight regulation.

描述（改编自申请人的摘要）： 本应用程序提出的研究是确定中央和 食物控制中的外围哺乳动物bommalian bombesin样肽 进气。 已经确定了两个哺乳动物BN样肽：胃蛋白 释放肽（GRP）和神经蛋白B（NMB）。 这些肽及其 相应的受体分布在 胃肠道以及中央和周围神经系统。 Bn like 肽抑制外围或中央给药后的食物摄入量 这些抑制似乎是模仿内源性的动作 肽由于GRP和NMB受体拮抗剂的给药增加了 食物摄入量。 提出的实验研究了： 1）外围和中央BN样的受体亚型介导 肽，不同； 2）外围的饱腹运动的调解 BN样肽取决于周围传入途径的激活 这导致GRP的尾后后脑释放； 3）中央 介导中央和外围BN样的饱腹感的途径 肽不同。 解决这些假设的实验将采用 特定的拮抗剂和反义寡核苷酸，C-FOS 免疫组织化学映射，周围和中央病变以及 微结构行为分析。 最后，调查人员建议 实验以鉴定哺乳动物BN样肽与 其他具有厌食症的肽，以更好地了解总体 BN样肽在食物摄入控制中的贡献。 一起， 这些实验的结果将显着增强我们的 了解大脑/肠肽在食物摄入和身体中的作用 体重调节。