NATIVE & RECOMB MEMBRANE PROTEIN TO DETERMINE FUNCT & DRUG DESIGN: HIV INTEGRASE

本国的

• 批准号: 6308872
• 负责人: Robert M Stroud
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308872
• 关键词: AIDS; bacteria; bioengineering /biomedical engineering; biological products; biomedical resource; biotechnology; carbohydrates; human tissue; immunology; infection; inflammation; lymphatic system; proteins; spectrometry; structural biology; technology /technique; virus

Our laboratory focus is to reveal functional mechanisms of integral membrane proteins at the level of chemistry and atomic structure for impact on drug design. Membrane proteins include bacteriorhodopsin, ATcase-bacteriorhodopsin fusion protein, nicotinic acetylcholine receptor, serotonin receptor, thrombin receptor, a NNa+/K= ATPase, and CHIP 28. Soluble proteins include thymidilate synthetase, creatine kinase, colicin, d-UMP hydroxymethylase, SIV/HIV protease, HIV integrase, B. thuringiensis toxins (Cry2a, Cry1c, Cyta), and a designed 4-helix bundle. Synthesized peptides include those used for antibody production, enzyme substrates and ligands, and peptide-based 'peptitergents' which assist in solubilization and crystallization of membrane proteins. Following isolation, expression or synthesis, these proteins and peptides will be purified and characterized prior to 3-D crystallization. To facilitate characterization and crystallization, mass spectrometry will be used to assay purity, mass, and post-translational processing events. In addition, these efforts will develop purification procedures of hydrophobic proteins and peptides in environments amenable for mass spectrometry.

我们实验室的重点是揭示功能机制 化学和原子水平的整合膜蛋白 对药物设计影响的结构。 膜蛋白包括 细菌视紫红质，ATcase-细菌视紫红质融合蛋白，烟碱 乙酰胆碱受体、血清素受体、凝血酶受体、 NNa+/K= ATP 酶和 CHIP 28。可溶性蛋白质包括胸苷 合成酶、肌酸激酶、大肠菌素、d-UMP 羟甲基酶、SIV/HIV 蛋白酶、HIV 整合酶、苏云金芽孢杆菌毒素（Cry2a、Cry1c、Cyta）、 和设计的 4 螺旋束。 合成的肽包括那些 用于抗体生产、酶底物和配体，以及 基于肽的“肽剂”，有助于溶解和 膜蛋白的结晶。 分离、表达后 或合成，这些蛋白质和肽将被纯化并 在 3-D 结晶之前进行表征。 为了方便 将使用表征和结晶、质谱分析 测定纯度、质量和翻译后加工事件。 在 此外，这些努力将开发纯化程序 适合质量的环境中的疏水性蛋白质和肽 光谱测定法。