CORRECTION OF HYPONATREMIA & PATHOGENESIS OF OSMOTIC INDUCED DEMYELINATION

纠正低钠血症

• 批准号: 6356299
• 负责人: SHELDON ADLER
• 金额: $ 3.8万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-20 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6356299
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; cognition; magnetic resonance imaging; nervous system; technology /technique

The long-term goal of this project is to apply Functional Magnetic Resonance imaging (fMRI) techniques to the awake behaving monkey so that powerful single unit recording techniques can be combined with more global views of brain function from MRI. The ability to correlate changes with direct electrical recordings will help to further clarify and extend the usefulness of fMRI. fMRI will have the potential to compensate for the two major limits of single-neuron recording in behaving monkeys - inability to survey the whole brain and inability to resolve detail at the level of cortical columns and patches. We have equipped a rhesus monkey with a cranial implant identical to those used for rigid head restraint during single-neuron recording. Activities during the preceding grant year have involved building basic apparatus and piloting fundamental methodology for the study of monkeys in the 4.7-T Bruker AVANCE-DRX. We constructed a cradle by which to support an anesthetized monkey in the 4.7-T scanner, with the head rigidly fixed inside the RF coil. In order to allow the monkey to hold the arms in a forward position during functional, we have designed and built a hemicylindrical RF coil with excellent RF homogeneity and sensitivity, which leaves free the space beneath the head. As a first step towards obtaining functional MR images of the monkey, using BOLD, we have detected a hemodynamic change in rhesus monkey cortex induced by a direct intervention (hypercapnia). We chose to manipulate blood flow with hypercapnia, rather than with brain activity, so as to circumvent the possible disruption, by anesthesia, of the functional linkage between brain activity and blood flow. This project will achieve a formal status of a TRD project begining next grant year, and experiments will continue towards carrying out fMRI in the awake monkey.

该项目的长期目标是应用功能磁性 共振成像（fMRI）技术对醒着的猴子的表现 强大的单个单元记录技术可以与 MRI对大脑功能的更多全球观点。 能力 将变化与直接电气记录相关联 进一步阐明并扩展了fMRI的实用性。 fMRI将有 补偿单神经元的两个主要限制的潜力 录制行为猴子 - 无法调查整个大脑 以及无法在皮质柱级别上解决细节和 补丁。 我们已经配备了恒河猴和颅内植入物 与单神经元期间用于刚性头部约束的固定 记录。 上一个赠款年度的活动涉及 建立基本设备和试点基本方法 4.7-T Bruker Avance-DRX中的猴子研究。 我们构建了一个 在4.7-T中支撑麻醉猴子的摇篮 扫描仪，头部牢固地固定在RF线圈内。 为了 允许猴子将手臂保持在前方位置 功能性，我们已经设计并建造了一个带有Hemicylindrical RF线圈 出色的RF同质性和敏感性，这留下了空间 在头部下方。 作为获得功能MR的第一步 猴子的图像，使用粗体，我们检测到了血液动力学 直接干预引起的恒河猴猴猴皮质的变化 （高碳酸盐）。 我们选择用高碳酸脂蛋白操纵血流， 而不是大脑活动，以规避可能的 通过麻醉的破坏大脑之间功能连锁 活性和血流。 该项目将获得正式地位 下一年开始的TRD项目，实验将继续 要在清醒猴子中进行fMRI。