Neurochemical organization of central chemosensory neurons

中枢化学感觉神经元的神经化学组织

基本信息

批准号：
6357122
负责人：
GINA M PETE
金额：
$ 29.24万
依托单位：
HOWARD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-01 至 2001-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6357122
关键词：
afferent nerve brain mapping chemoreceptors cooperative study hypercapnia immediate early protein immunocytochemistry laboratory rat neurochemistry neuronal transport neurotransmitter receptor protooncogene respiration regulatory center respiratory function sensorimotor system

项目摘要

This proposal consists of research focused on: 1) the central neurochemical organization of sensory neurons that triggers integrated respiratory responses to changes in CO2/H+, 2) the receptor systems of these neurons, activation of which may modulate their response to CO2/H+ signal, and 3) their connectivity with respiratory output neurons such as hypoglossal motor cells. Recently, it has been shown that expression of immediate-early genes are sensitive, inducible, and broadly applicable markers for neurons activated by extracellular stimuli. In the proposed studies, we will use expression of c-fos gene encoded protein (cFos) to identify neurons activated by hypercapnia, in order to investigate neurotransmitter content and receptor systems of these cells. The connectivity of chemosensory neurons with the respiratory-related motoneuron pools will be determined by combining retrograde tracer technique with cFos immunohistochemistry. The data derived from these studies will provide basic information related to the central chemosensory network, neurochemical organization, and chemical nature of communications between chemosensitive ells and brainstem output neurons. These results will have indirect bearing on the understanding of neurochemical mechanisms underlying centrally mediated respiratory and cardiovascular dysfunctions, as might occur in idiopathic alveolar hypoventilation, sleep apnea and sudden infant death syndrome.

该提案由重点研究的研究组成：1）感官神经元的中央神经化学组织，这些神经化学神经元的中央神经化学组织触发对CO2/H+的变化的整合呼吸反应，2）这些神经元的受体系统的激活可能会调节其对CO2/H+信号的反应，以及3）它们与呼吸输出神经元的连通性，例如Hypog ossal hypoglossal Motylossal Motion Motors。最近，已经表明，直接至上的基因的表达是敏感的，诱导的且广泛适用于由细胞外刺激激活的神经元的标记。在拟议的研究中，我们将使用C-FOS基因编码蛋白（CFO）的表达来鉴定高碳酸脂蛋白激活的神经元，以研究这些细胞的神经递质含量和受体系统。化学感应神经元与呼吸相关的运动神经元池的连通性将通过将逆行示踪技术与CFOS免疫组织化学相结合来确定。这些研究得出的数据将提供与中央化学感应网络，神经化学组织以及化学敏感性ELL和脑干输出神经元之间通信的化学性质有关的基本信息。这些结果将与理解中心介导的呼吸道和心血管功能障碍的神经化学机制的理解有关，这可能是特发性肺泡过度衰减，睡眠呼吸暂停和猝死综合征。