INDEPENDENT SCIENTIST AWARD (NIEHS)

独立科学家奖 (NIEHS)

• 批准号: 6382039
• 负责人: Ashis K Basu
• 金额: $ 8.43万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6382039
• 关键词: DNA damage; DNA repair; DNA replication; Escherichia coli; animal genetic material tag; bacterial genetics; bacteriophage M13; carcinogen testing; chemical carcinogen; chemical structure function; fish; genetically modified animals; guanosine; microorganism culture; mutagen testing; mutagens; nitrates; nucleic acid sequence; purine analog; pyrenes; virus genetics

The objective of this current research is to investigate the mechanisms of mutagenesis and DNA repair induced by the nitrated pyrenes. To accomplish this goal, his laboratory has used the tools of organic synthesis and recombinant DNA technology to construct specific DNA fragments and viral genomes that contained, at pre-selected sites, the major DNA adduct formed by 1-nitropyrene. This technology will be further employed to construct specific mutagenic hot-spot sequences in DNA. Sequence-specificity of mutagenesis will be investigated by introducing these modified viral genomes in Escherichia coli cells,, where the DNA replication and repair systems will act upon the single DNA adduct. A primary goal of this work is to define the relationship between the structure and three-dimensional effects of a lesion in DNA and the mutagenicity and DNA repair that it may induce. Another important aim is to understand the mechanism of how the repair proteins protect cells against the toxic and mutagenic properties of these DNA damaging agents.

当前研究的目的是调查其机制 硝化芘诱导的诱变和 DNA 修复。为了完成 为了这个目标，他的实验室使用了有机合成和 重组DNA技术构建特定的DNA片段和病毒 基因组在预先选择的位点包含形成的主要 DNA 加合物 由1-硝基芘。该技术将进一步用于构建 DNA 中特定的诱变热点序列。序列特异性 将通过引入这些修饰的病毒来研究诱变 大肠杆菌细胞中的基因组，其中 DNA 复制和修复 系统将作用于单个 DNA 加合物。这项工作的主要目标 就是定义结构和三维之间的关系 DNA 损伤的影响及其可能产生的致突变性和 DNA 修复 诱导。另一个重要目标是了解 修复蛋白保护细胞免受毒性和诱变特性的影响 这些 DNA 损伤剂。