BIOGENIC AMINE SYSTEMS, CRF, AND STRESS

生物胺系统、CRF 和压力

• 批准号: 6228975
• 负责人: RITA VALENTINO
• 金额: $ 12.38万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6228975
• 关键词: antidepressants; autoradiography; biological signal transduction; corticotropin releasing factor; dorsal raphe nucleus; hormone receptor; immunocytochemistry; laboratory rat; locus coeruleus; neural plasticity; neural transmission; neurotransmitters; psychopharmacology; stress

DESCRIPTION (applicant's abstract): This is a proposal for a Senior Scientist Award (K05) from an investigator who has been funded by a Research Scientist Development Award (K02) for the last 10 years. Prior K02 funding advanced the PI's career by providing time to develop new approaches and foster productive collaborations. At a research level this resulted in expansion from a single to 3 major interrelated NIH-funded research programs. At a technical level, expertise went from predominantly in vivo electrophysiological approaches to including in vivo microdialysis, as well as sophisticated systems and cellular neuroanatomical techniques. At a career level, the PI established a Division of Stress Neurobiology within the Department of Psychiatry [at Hahnemann University]. In February the Division moved to the Children's Hospital of Philadelphia where the PI was offered a prestigious position as a Stokes Investigator and faculty position at the University of Pennsylvania. The proposed research plan integrates aspects of the 3 funded research programs to address the global hypothesis that the impact of corticotropin-releasing factor on biogenic amine systems is integral in the acute stress response and that stress-induced plasticity of these interactions underlies certain stress-related psychopathology. The proposed career development plan will allow the PI to develop and incorporate new cellular approaches into the experimental repertoire of systems approaches currently used to elucidate CRF-biogenic amine interactions and mechanisms underlying stress-induced plasticity. These include cellular and molecular electrophysiological techniques in in vitro slice preparations and the use of viral vectors to manipulate CRF expression in specific CRF afferents to biogenic amine nuclei. These techniques will arm the PI with a range of tools necessary to address research questions on multiple levels (from cellular to systems). A second aspect of career development is to mobilize basic and clinical investigators within the CHOP/ Upenn community who share a common interest in stress in a Center for Stress Neurobiology. Electronic communications, regular research seminars, retreats and shared common resources are used to foster interactions and collaborations among faculty. Because the Center will focus on stress as an etiological factor, rather than on a specific disease, it promises to be an ideal mechanism for understanding and developing new treatments for diverse medical and behavioral disorders. Like the previous K02 award, the K05 award will promote future career development by providing stable salary support and flexibility to enhance technical skills and conceptual bases and promoting leadership.

描述（申请人的摘要）：这是高级科学家的建议 由研究科学家资助的调查员的奖项（K05） 过去10年的发展奖（K02）。先前的K02资金提高了 Pi的职业生涯通过提供时间来开发新方法并促进生产力 协作。在研究层面上，这导致了一个单一的扩展 3主要相互关联的NIH资助研究计划。在技​​术层面 专业知识主要是从体内电生理学方法到 包括体内微透析以及复杂的系统和细胞 神经解剖技术。在职业层面，PI建立了一个部门 精神病学系内的压力神经生物学[在Hahnemann 大学]。 2月，该师搬到了儿童医院 PI被享有盛名的位置作为Stokes的费城 宾夕法尼亚大学的研究人员和教职员工。这 拟议的研究计划将3个资助研究计划的各个方面融合到 解决了全球假设，即皮质激素释放因子的影响 关于生物胺系统在急性应力反应中是不可或缺的，并且 这些相互作用的应力诱导的可塑性是一定的 与压力有关的心理病理学。拟议的职业发展计划将允许 PI开发并将新的细胞方法纳入实验 目前用于阐明CRF生物胺的系统方法的曲目 应力诱导的可塑性的相互作用和机制。这些包括 体外切片中的细胞和分子电生理技术 制备和使用病毒载体操纵CRF表达 生物胺核的特定CRF传入。这些技术将武装 PI带有一系列工具，以解决有关多个的研究问题 水平（从细胞到系统）。职业发展的第二个方面是 动员Chop/ Upenn社区中的基本和临床研究人员 在压力神经生物学中心中对压力有共同的兴趣。 电子通信，定期研究研讨会，务虚会和共享 共同资源用于促进互动和合作 学院。因为中心将专注于应力作为病因因素，所以 而不是针对特定疾病，它有望成为理想的机制 了解和开发针对多种医学和行为的新治疗 疾病。像以前的K02奖一样，K05奖将促进未来 通过提供稳定的工资支持和灵活性来发展职业发展 提高技术技能和概念基础并促进领导力。