COMPLETE GENOME SEQUENCE OF RICKETTSIA TYPHI

伤寒立克次体全基因组序列

• 批准号: 6299108
• 负责人: DAVID H WALKER
• 金额: $ 30.77万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299108
• 关键词: DNA; Rickettsia; bacteria infection mechanism; bacterial genetics; bioterrorism /chemical warfare; genetic mapping; genetic techniques; genome; molecular biology information system; nucleic acid sequence; virulence

The complete DNA sequences of the genome of Rickettsia typhi will be determined and analyzed for putative coding regions and possible functions of the gene products. Under an addendum for potential bioterrorism agents of the Policy on Support of Large- Scale Genome Sequencing Projects, R. typhi has been designated as a priority bioterrorism agent. This application is prepared in response to this opportunity for support of large-scale genome sequencing of high priority pathogens as potential agents of bioterrorism. Rickettsia typhi is highly infectious, including via aerosol exposure, infection is deceptively difficult to diagnose clinically and causes a severe, incapacitating illness with 1 percent overall mortality. Knowledge of its genome would be most useful for comparison with that of the more virulent R. prowazekii and for investigation of virulence factors, targets of attenutation, and potential novel targets for antimicrobial therapy of strains that could be engineered to resistance to the only two effective agents, tetracyclines and chloramphenicol. Once the genomic sequences are determined and analyzed they will be made available to the research community through an online database with a number of tools to facilitate data retrieval. During the course of the project there will also be ongoing release of preliminary data.

将确定伤寒立克次体基因组的完整 DNA 序列并分析其推定的编码区和基因产物的可能功能。 根据支持大规模基因组测序项目政策的潜在生物恐怖分子附录，伤寒杆菌已被指定为优先生物恐怖分子。 本申请是为了响应支持对作为生物恐怖主义潜在媒介的高优先级病原体进行大规模基因组测序的机会而准备的。 伤寒立克次体具有高度传染性，包括通过气溶胶暴露，感染在临床上很难诊断，并会导致严重的、致残的疾病，总死亡率为 1%。 了解其基因组对于与毒性更强的普瓦泽基立克氏菌的基因组进行比较、研究毒力因子、减毒靶点以及菌株抗菌治疗的潜在新靶点最为有用，这些菌株可以被设计为对仅有的两种有效的耐药菌株。剂、四环素类和氯霉素。一旦基因组序列被确定和分析，它们将通过在线数据库提供给研究界，并提供多种工具来促进数据检索。在项目过程中，还将不断发布初步数据。