WHOLE GENOME SEQUENCING OF COXIELLA BURNETTI

伯内蒂柯克斯体全基因组测序

• 批准号: 6298818
• 负责人: JOHN F HEIDELBERG
• 金额: $ 60.76万
• 依托单位: INSTITUTE FOR GENOMIC RESEARCH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2002-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6298818
• 关键词: Q fever; Rickettsiales; bacteria infection mechanism; bacterial vaccines; computer assisted sequence analysis; genetic library; genetic mapping; genetic techniques; genome; microorganism disease chemotherapy; nucleic acid sequence; polymerase chain reaction; vaccine development; virulence; zoonosis

The zoonotic disease Q fever is a caused by the obligate intracellular bacterium Coxiella burnetii. Coxiella burnetii has evolved strategies to survive in the hostile phagolysosomal environment. This agent is important for developing a comparative perspective on bacterial pathogenesis strategies. Most pathogens either do not survive extended periods in the environment or, like many Gram-positive agents, have evolved the ability to sporulate to survive long periods of dessication. Coxiella burnetii is a Gram-negative organisms that has developed a novel, non-sporulating strategy to survive in a non-replicative form for long periods. This property has lead to the consideration of this pathogen as a bio-warfare agent. Its stability in the environment presents a serious concern for officials responsible for planning a response to the illegitimate release of this agent. Elucidation of the complete genomic sequence would provide a major breakthrough in the design of therapeutic intervention and a unique perspective on its biology. We propose to sequence the 2.1 Mbp genome of Coxiella burnetii Nine Mile, phase I (RSA 493) because of its known virulence potential and subsequent potential for comparison with other isolate groups. The genome sequence will be accomplished by the whole genome random shotgun sequencing currently used at TIGR. Additionally, the genome will be annotated by computer techniques to identify all open reading frames (ORFs) and relate as many ORFs as possible to proteins of known function.

人畜共患病Q发烧是由强制性细胞内细菌Coxiella burnetii引起的。 Coxiella burnetii已经发展了策略，以在敌对的吞噬物质环境中生存。 该药物对于开发细菌发病机理的比较观点很重要。大多数病原体要么无法在环境中长期存活，要么像许多革兰氏阳性药物一样，已经发展出孢子虫的能力，可以长期生存。 Coxiella burnetii是一种革兰氏阴性的生物，它已经开发出一种新型的非散发策略，可长期以非复制形式生存。 该特性导致将这种病原体视为生物 - 雷刺剂。 它在环境中的稳定性对负责计划对该代理非法释放的官员表示了严重关注。 阐明完整的基因组序列将在治疗干预的设计以及对其生物学的独特视角上取得重大突破。我们建议对Coxiella burnetii 9英里（RSA 493）的2.1 MBP基因组进行测序，因为其已知的毒力潜力和随后与其他分离株的比较潜力。 基因组序列将通过目前在TIGR上使用的整个基因组随机shot弹枪测序来完成。此外，将通过计算机技术来注释基因组，以识别所有开放式阅读帧（ORF），并将尽可能多的ORF与已知功能的蛋白质联系起来。