ORTHOPOXVIRUS GENOMICS % BIOINFORMATICS RESOURCE CENTER

正痘病毒%20基因组学%20%%20生物信息学%20资源%20中心

• 批准号: 6229304
• 负责人: Robert MARK Buller
• 金额: $ 49.82万
• 依托单位: SAINT LOUIS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-27 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6229304
• 关键词: Internet; Poxviridae; Poxviridae disease; cooperative study; informatics; microorganism resource /registry; molecular biology information system; virus genetics

Of the potential organisms that might be used in bioterrorism, smallpox would likely have the most horrific impact on the health of the U.S. Small pox is highly Contagious and mortality is in the range of 30%. There is no available antiviral therapy. Furthermore, the U.S. population is essentially entirely susceptible because routine immunization was terminated in the early 1960's. Even older vaccines are vulnerable as vaccine protection wanes significantly after ten years. As a result of the eradication of the disease, and with the exception of the development of fowlpox vaccine vectors, the amount of research on smallpox and related poxviruses has decreased significantly. This proposal will support the acquisition and analysis of orthopoxvirus genomic information to facilitate its application to the development of therapies and improved vaccines for smallpox and related viruses. The goals of the project are: 1) Collect and/or determine the genomic sequences of variola virus isolates and orthopoxviruses that might serve as surrogates for variola virus in animal models; 2) Design and maintain a relational database to store, display, annotate and query genome sequence, structural information, phenotypic data (e.g. drug susceptibility, virulence, host specificity) and bibliographic information; 3) Apply innovative methods for global functional analysis of the viral genomes; 4) Serve as a repository of well-documented viral strains (including clinical isolates and mutants, if relevant) and genomic clones that are used in the genomic sequence analysis; and 5) Develop and maintain a website.

在可能用于生物恐怖主义的潜在生物体中，天花可能对美国的健康产生最可怕的影响。天花具有高度传染性，死亡率在 30% 左右。目前尚无可用的抗病毒疗法。此外，由于常规免疫接种于 20 世纪 60 年代初终止，美国人基本上完全易感。即使是较旧的疫苗也很容易受到攻击，因为十年后疫苗的保护作用会显着减弱。由于疾病的根除，除了鸡痘疫苗载体的开发外，对天花和相关痘病毒的研究量显着减少。该提案将支持正痘病毒基因组信息的获取和分析，以促进其应用于天花及相关病毒的疗法和改进疫苗的开发。该项目的目标是： 1) 收集和/或确定天花病毒分离株和正痘病毒的基因组序列，这些序列可能作为动物模型中天花病毒的替代品； 2）设计和维护关系数据库，用于存储、显示、注释和查询基因组序列、结构信息、表型数据（例如药物敏感性、毒力、宿主特异性）和书目信息； 3）应用创新方法对病毒基因组进行全局功能分析； 4) 作为有据可查的病毒株（包括临床分离株和突变体，如果相关）和用于基因组序列分析的基因组克隆的存储库； 5) 开发和维护网站。