INFANT CHEMOKINE PRODUCTION AND SUSCEPTIBILITY TO HIV INFECTION

婴儿趋化因子的产生和艾滋病毒感染的易感性

• 批准号: 6305968
• 负责人: HENRY POLLACK
• 金额: $ 2.1万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6305968
• 关键词: chemokine; clinical research; cytokine receptors; developmental immunology; disease /disorder etiology; disease /disorder proneness /risk; human immunodeficiency virus; human subject; infant human (0-1 year); microorganism immunology; pathologic process; pediatric AIDS; protein isoforms; receptor expression

This proposal will examine some of the potential determinants of perinatal transmission of HIV. Recently it was shown that the susceptibility of CD4+ T cells to HIV infection is related to both the quantity of b chemokine (MIP-1a, MIP-1 b and RANTES) produced by their CD4+ T cells and to the expression of the b chemokine receptor 5 (CCR5). This receptor was recently identified as the co-receptor for monocytotropic (M-tropic) isolates of HIV, which are the viruses that are mainly transmitted heterosexually and perinatally. Individuals who have homozygous mutations of the CCR5 receptor are resistant to HIV infection with M-tropic isolates. We will examine whether there is a correlation between either CCR5 expression or chemokine production and perinatal HIV transmission. As these chemokines also mediate CD8+ T-cell-mediated HIV suppression, which we have identified as an important early protective immune response in HIV-infected infants, we will also measure CD8 viral suppressive activity (CD8-VSA) to try to assess whether it also plays a role in perinatal HIV transmission. The laboratory was used for oligonucleotide synthesis, and recombinant DNA techniques.

该提案将检查艾滋病毒围产期传播的一些潜在决定因素。 最近显示，CD4+ T细胞对HIV感染的敏感性与B趋化因子的量（MIP-1A，MIP-1 B和RANTES）的量有关，其CD4+ T细胞产生的量以及B趋化因子受体5（CCR5）的表达。 该受体最近被鉴定为HIV的单核（M-胶质）分离株的共受体，它们是主要和围产期传播的病毒。 具有CCR5受体纯合突变的个体对使用M冰球分离株具有抗HIV感染。 我们将检查CCR5表达或趋化因子产生与围产期HIV传播之间是否存在相关性。 由于这些趋化因子还介导CD8+ T细胞介导的HIV抑制作用，在HIV感染的婴儿中，我们已确定为重要的早期保护性免疫反应，我们还将测量CD8病毒抑制活性（CD8-VSA），以评估它在催生HIV传播中是否也起作用。该实验室用于寡核苷酸合成和重组DNA技术。