CORE--LABORATORY ANIMAL

核心--实验动物

• 批准号: 6299420
• 负责人: HARRY VAN STEEG
• 金额: $ 17.7万
• 依托单位: CTRC RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-15 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299420
• 关键词: DNA damage; DNA repair; aging; animal genetic material tag; animal tissue; biological models; biomedical facility; cell bank /registry; cell line; cell senescence; gene mutation; genetically modified animals; histopathology; laboratory mouse

Both scientific and economic considerations make it desirable that the transgenic and control mice to be used in this program are house dat one central location. With respect to scientific considerations, the correlation of DNA repair deficiencies in different transgenic lines with spontaneous DNA damage, mutations and cellular senescence, necessitates the exclusion as much as possible of environmental variation. Hence, the need to wean and house the animals under uniform and well-controlled conditions. Economic considerations involved the cost-effectiveness of sharing organs/tissues,c ell lines and DNA samples. The Transgenic Animal Core will provide investigators of the three projects with all these biological materials upon request. It will import mutant and transgenic animals into the RIVM barrier facility, breed all transgenic lines into C57/BL/6, make the necessary crosses among the different lines, establish aging cohorts and determine survival of the individual transgenic lines, and perform complete histopathological examination of all organs and tissues.

科学和经济方面的考虑使得在该计划中使用的转基因和控制小鼠是房屋中心位置。就科学考虑而言，不同转基因线上DNA修复缺陷与自发DNA损伤，突变和细胞衰老的相关性需要尽可能地排除环境变化。因此，需要在统一且控制良好的条件下断奶和容纳动物。经济考虑因素涉及共享器官/组织，C ELL系和DNA样本的成本效益。转基因动物核心将根据要求向研究人员提供所有这些生物材料。它将将突变动物和转基因动物进口到RIVM屏障设施中，将所有转基因线繁殖为C57/BL/6，使不同的线之间的必要杂交，建立老化队列并确定单个转基因线的存活，并对所有器官和组织进行完整的组织病理学检查。