ANTI-HIV MICROBICIDES IN CELLS AND TISSUES

细胞和组织中的抗 HIV 杀菌剂

• 批准号: 6352616
• 负责人: Phalguni GUPTA
• 金额: $ 5.83万
• 依托单位: MAGEE-WOMEN'S HOSPITAL OF UPMC
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-15 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6352616
• 关键词: AIDS education /prevention; HIV infections; acid base balance; antiAIDS agent; antibiotics; cervix; clinical research; drug interactions; genital secretion; human immunodeficiency virus 1; human tissue; lymphocyte; organ culture; pharmacokinetics; sexually transmitted diseases; topical drug application; vagina

There is an urgent need for the development of vaginal microbicide that inhibit HIV transmission. An ideal antiHIV vaginal microbicide would inhibit viral infection from cell-free and cell-associated HIV with varying biologic properties, and be non-toxic for cells and vaginal and epithelium tissues. The overall objective of the project is to develop procedures to evaluate these anti-HIV properties of vaginal microbicide. Specific aims of the project are: a) To evaluate anti-HIV activity of topical microbicide against cell-free and cell-associated HIV in lymphocytes and T cell lines, b) To test the effect of environmental factors, such as low pH, semen, vaginal secretions on the anti-HIV activity of the vaginal microbicide, c) To examine the cytopathic effect of anti-HIV microbicide on the vaginal and cervical tissues using an organ culture system, d) To test the effect of microbicide on the transmission of HIV across the cervical tissue using an organ culture model. Such information will be extremely useful in development of vaginal microbicide that can prevent sexual transmission of HIV.

迫切需要开发抑制艾滋病毒传播的阴道杀微生物剂。理想的抗艾滋病毒阴道杀微生物剂能够抑制具有不同生物学特性的无细胞和细胞相关艾滋病毒的病毒感染，并且对细胞、阴道和上皮组织无毒。该项目的总体目标是开发评估阴道杀菌剂的抗艾滋病毒特性的程序。该项目的具体目标是：a) 评估局部杀菌剂对淋巴细胞和 T 细胞系中无细胞和细胞相关 HIV 的抗 HIV 活性，b) 测试环境因素的影响，例如低 pH 值、精液，阴道分泌物对阴道杀菌剂的抗 HIV 活性的影响，c) 使用器官培养系统检查抗 HIV 杀菌剂对阴道和宫颈组织的细胞病变作用，d) 测试使用器官培养模型研究杀微生物剂对艾滋病毒在宫颈组织传播的影响。这些信息对于开发可以预防艾滋病毒性传播的阴道杀微生物剂非常有用。