CORE--MORPHOLOGY AND TISSUE CULTURE

核心——形态学和组织培养

• 批准号: 6112267
• 负责人: JOHN RICHARD SLADEK
• 金额: $ 20.03万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6112267
• 关键词: Cercopithecidae; Parkinson's disease; biomedical facility; brain; computer simulation; dopamine; histology; immunocytochemistry; immunofluorescence technique; methylphenyltetrahydropyridine; nervous system transplantation; tissue /cell culture

The morphology and tissue culture core will provide histological and tissue culture support for all three projects. Immunohistochemistry for tyrosine hydroxylase will be used as a primary marker for dopaminergic neurons. Quantitation of dopamine neuron numbers in grafted tissue and in the substantia nigra of MPTP treated monkeys will be assessed for each relevant experiment. Computer assisted three-dimensional reconstructions will be made, particularly for attempts at circuit reconstruction using striatal- nigral co-grafts. Additional morphological methods and techniques provided by this core and its personnel are cryopreservation, fluorescence histochemistry, riboprobe in situ hybridization for tyrosine hydroxylase, immunochemistry for a number of other transmitters. Core personnel perform all fetal brain dissections and participate in adult brain dissections and micropunches that rely upon a combined histochemical/biochemical procedure. The tissue culture facility will carry out pharmacologic studies described in Project 1.

形态和组织培养核心将提供组织学和组织 对这三个项目的文化支持。 酪氨酸的免疫组织化学 羟化酶将用作多巴胺能神经元的主要标记。 嫁接组织中多巴胺神经元数量的定量 将对每种相关的MPTP处理过的猴子的黑质进行评估 实验。 计算机辅助三维重建将是 制作，特别是用于尝试使用纹状体进行回路重建的尝试 nigral共移植。 提供的其他形态学方法和技术 核心及其人员是冷冻保存，荧光 组织化学，核探针原位杂交酪氨酸羟化酶， 许多其他发射机的免疫化学。 核心人员表演 所有胎儿脑解剖并参与成人脑部解剖和 依赖于组织化学/生化程序的合并的微置。 组织培养设施将进行描述的药理研究 在项目1中。