Core--Molecular diagnostics

核心--分子诊断

• 批准号: 6211799
• 负责人: DANIEL PINKEL
• 金额: --
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-05 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6211799
• 关键词: biomedical facility; confocal scanning microscopy; cytogenetics; flow cytometry; gene expression; genetic techniques; in situ hybridization; linkage mapping; messenger RNA; neoplasm /cancer diagnosis; nucleic acid sequence; polymerase chain reaction

Description: (Applicant's Description) The Molecular Diagnostics Core provides Cancer Center members with access to technical capability that is not available to individual laboratories. The Core provides established techniques, offers new procedures developed in Cancer Center Research Programs, and facilitates clinical translation of newly developed tests. The Molecular Diagnostics Core contains 4 components: Cytometry: Provides flow cytometry and cell sorting, image cytometry including multi-color quantitative fluorescence microscopy, data analysis, confocal microscopy, and education. Genome Analysis: Provides DNA sequencing, analysis of LOH, linkage, SSCP, and microsatellite instability, and quantitative PCR analysis using TaqMan. Molecular Cytogenetics: Provides fluorescence in situ hybridization (FISH) based capability including probe mapping, copy number and translocation analysis, and comparative genomic hybridization (CGH) for human and murine specimens. It also provides laser capture microdissection, technical consultation, and education. Microarrays: Will provide DNA microarray capability for analysis of genomic copy number changes and mRNA expression levels. Each component of the Molecular Diagnostics Core is directed by a Cancer Center faculty member and operated by a manager. An advisory board consisting of a rotating group of three Cancer Center Program leaders and a representative from each of the departments of Laboratory Medicine and Pathology will oversee core operation and development to assure that resources are apportioned most effectively among the components to meet the needs of the Cancer Center investigators, and that the activities of this core are appropriately complementary to the other Cancer Center cores. Support for the Genome Analysis and Molecular Cytogenetics Shared Resources is requested as part of the CCSG; support for the Microarrays Service is requested under Developmental Funds; and the Cytometry Core is sustained by institutional support.

描述：（申请人的描述）分子诊断核心为癌症中心成员提供了无法为个别实验室提供的技术能力。 核心提供了既定的技术，提供了在癌症中心研究计划中开发的新程序，并促进了新开发的测试的临床翻译。分子诊断核心包含4个组件：细胞仪：提供流式细胞术和细胞分类，图像细胞仪，包括多色定量荧光显微镜，数据分析，共聚焦显微镜和教育。基因组分析：使用Taqman提供DNA测序，LOH分析，连锁，SSCP和微卫星不稳定性以及定量PCR分析。分子细胞遗传学：提供基于原位杂交（FISH）的荧光能力，包括探针映射，拷贝数和易位分析以及对人和鼠标本的比较基因组杂交（CGH）。 它还提供激光捕获微分解，技术咨询和教育。微阵列：将提供DNA微阵列能力，以分析基因组拷贝数变化和mRNA表达水平。 分子诊断核心的每个组成部分均由癌症中心教职员工指导，并由经理操作。 由由三个癌症中心计划领导者组成的旋转组组成的顾问委员会以及每个实验室医学和病理部门的代表将监督核心运营和开发，以确保资源在癌症中心调查人员的需求中最有效地分配资源，以满足该核心的活动，并且与其他癌症中心的活动适当地互补。支持基因组分析和分子细胞遗传学共享资源作为CCSG的一部分。在开发基金下要求对微阵列服务的支持；并且细胞术核心由机构支持维持。