CULTURED CILIATED CELLS FOR HIGH THROUGHPUT SCREENING

用于高通量筛选的培养纤毛细胞

基本信息

批准号：
6338215
负责人：
Hua Mao
金额：
$ 10.59万
依托单位：
BIOTECHPLEX CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-30 至 2002-03-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6338215
关键词：
bioreactors cell line cilium cilium /flagellum motility collagen growth media high throughput technology laboratory rabbit respiratory epithelium technology /technique development tissue /cell culture

项目摘要

DESCRIPTION (Applicant's Abstract): BioTechPlex proposes to develop a mammalian, tracheal/bronchial airway epithelial cell line specifically for high throughput screening. This is based on physiological functional assay. These cells will have a minimum of 3-6 weeks life-span following secondary culture. The outcome of this project provides the first cell model system that can be used for a new generation of HTS platform for pulmonary drug discovery as well as serving the specific needs for cellular physiology/pharmacology research and tissue engineering. In this Phase I project, BioTechPlex will develop a technique to culture the airway epithelial cells such that their ciliary functions are preserved longer than 16 weeks and sub-culture these cells either in collagen-coated submerged medium or using an air-liquid interface. The investigators will develop a technique to reactivate the dormant ciliogenesis mechanisms to regenerate the cilia in these epithelial cells with a minimum of 50 percent of the cells are ciliated, a condition in close resemblance to that found in vivo and in native tracheal and major bronchial epithelia. In Phase II, BioTechPlex will construct a rotating bio-reactor system to produce large quantities of these airway ciliated epithelial cells of the major mammalian species used in epithelial research and drug screening. PROPOSED COMMERCIAL APPLICATION: Not Available

描述（申请人的摘要）：BioTechPlex 提议开发一种哺乳动物气管/支气管气道上皮细胞系，专门用于高通量筛选。这是基于生理功能测定。这些二次培养后细胞的寿命至少为 3-6 周。该项目的成果提供了第一个可用于也用于肺部药物发现的新一代 HTS 平台满足细胞生理学/药理学研究的特定需求和组织工程。在这个第一阶段项目中，BioTechPlex 将开发一种培养气道上皮细胞的技术，使其纤毛功能保存时间超过 16 周，并对这些细胞进行传代培养在胶原蛋白包被的浸没介质中或使用气液界面。这研究人员将开发一种技术来重新激活休眠的纤毛发生在这些上皮细胞中再生纤毛的机制最少 50% 的细胞有纤毛，这种情况与此非常相似存在于体内以及天然气管和主要支气管上皮中。同相 II、BioTechPlex将建造一个旋转生物反应器系统来生产大量主要哺乳动物气道纤毛上皮细胞的数量用于上皮研究和药物筛选的物种。拟议的商业应用：无法使用