CORE--ANIMAL

核心--动物

• 批准号: 6316957
• 负责人: Robert L Jilka
• 金额: $ 19.64万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316957
• 关键词: animal breeding; animal care; animal colony; biomedical facility; cryopreservation; embryo /fetus preservation; genetically modified animals; information systems; laboratory mouse; male castration; ovariectomy; photon absorptiometry; urinalysis

The goals of the Animal Facility Core are to provide well-defined SAMR1, SAMP6 and IL-6 knockout mice for the investigators of the program project, and to assist investigators in the design and conduct of animal studies. This Core will be a cost-effective means of properly maintaining precious breeding stocks of mice that are essential for the conduct of experiments proposed by investigators in the program project. Economy will also be served by pooling requests for animals to be used in experiments. The Core will also be a centralized source for information on individual mice maintained by the facility. To achieve these goals the Animal Facility Core will perform the following. 1. Maintain a database on mice in the colon. Each animal will be electronically tagged at weaning. Information such as the date of birth, weight at weaning, identity of parents, and other features obtained by measurement or observation, will be recorded in a computerized database. This information will be available to each investigator and will be used by the Core Leader, in conjunction with project leaders, to select animals for experiments. 2. Maintain inbred mouse strains and assess genotype of each strain. Foundation stocks of SAMR1, SAMP6 and IL-6 knockout mice will be established; and production colonies of each strain will be set up as needed to produce animals for the conduct of experiments by investigators in the program project. For each colony, the Core will select breeders to minimize strain divergence. To assure strain authenticity, the genotype of SAMR1 and SAMP6 mice will be monitored by determining the H-2K haplotype, or by analysis of sequence length polymorphic microsatellite markers that uniquely identify the genome of each strain. In the case of the IL-6 knockout colony, genotype will be assessed by detection of the truncated IL-6 gene by Southern blotting. In conjunction with Jackson Laboratories (Bar Harbor, ME) embryos of the SAMP6 and SAMR1 mouse strains will be cryogenically preserved so that each strain can be reconstituted in case the breeding stock is lost. Finally, the overall health of each colony will be assessed periodically by necropsy. 3. Assist in conduct of animal experiments. Animal Facility Core personnel will conduct routine procedures in coordination with personnel in the Division of Laboratory Animal Medicine of UAMS. These include surgical procedures, administration of reagents or experimental test materials to mice, preparation of anesthetized animals for determination of bone mineral density by dual energy X-ray absorptiometry, and collection of serum and urine samples.

动物设施核心的目标是提供明确定义的SAMR1， SAMP6和IL-6淘汰小鼠该计划项目的调查人员， 并协助研究人员设计和进行动物研究。 该核心将是适当维护宝贵的一种经济高效的手段 小鼠的繁殖库存对于实验的进行至关重要 调查人员在计划项目中提出。 经济也将是 通过合并用于实验的动物的请求。 这 核心也将是单个小鼠信息的集中式来源 由设施维护。 为了实现这些目标，动物设施 核心将执行以下操作。 1。在小鼠上保持数据库 冒号。 每只动物将在断奶时以电子方式标记。 信息 例如出生日期，断奶的体重，父母的身份以及 通过测量或观察获得的其他功能将记录在 一个计算机数据库。 此信息将可用于每个信息 调查员，核心领导者将与 项目领导者，选择动物进行实验。 2。维持近交 小鼠菌株并评估每种菌株的基因型。 基础库存 将建立SAMR1，SAMP6和IL-6敲除小鼠；和生产 每种菌株的菌落将根据需要建立 计划项目中调查人员的实验进行。 为了 每个菌落，核心将选择育种者，以最大程度地减少应变差异。 为了确保应变真实性，SAMR1和SAMP6小鼠的基因型将 通过确定H-2K单倍型或通过序列分析来监测 长度多态性微卫星标记，独特地识别 每个菌株的基因组。 在IL-6敲除菌落中，基因型 将通过Southern检测到截短的IL-6基因来评估 吸毒。 与杰克逊实验室（我的巴港）结合 SAMP6和SAMR1小鼠菌株的胚胎将是低温的 保留，以便在繁殖时可以重组每个应变 股票丢失。 最后，每个殖民地的整体健康将是 定期通过尸检评估。 3。协助动物行为 实验。 动物设施核心人员将进行例行 与实验室部人员协调的程序 UAM的动物医学。 这些包括手术程序， 对小鼠的试剂或实验测试材料的给药， 制备麻醉动物以测定骨矿物质 双能X射线吸收法的密度，血清的收集和收集 尿液样品。