PATHOGENESIS OF ALEUTIAN MINK DISEASE PARVOVIRUS INFECTIONS

阿留申水貂病细小病毒感染的发病机制

• 批准号: 6288792
• 负责人: Marshall Elliott Bloom
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6288792
• 关键词: Carnivora; Parvoviridae; antibody formation; antiviral antibody; capsid; communicable disease diagnosis; cytokine; host organism interaction; immunoelectrophoresis; laboratory rabbit; microorganism culture; microorganism immunology; nucleic acid sequence; pathologic process; polymerase chain reaction; virus antigen; virus protein

Persistent infection of adult mink with the Aleutian mink disease parvovirus (ADV) leads to disturbances of immune regulation, including hypergammaglobulinemia, plasmacytosis, immune complex disease, interstitial and glomerulonephritis. The exceptionally high levels of antiviral antibody are not able to eliminate the virus, and in fact enhance infection of Fc-receptor bearing cells, like macrophages. We are studying ADV infection in vivo, in vitro and in an ex vivo system of gel supported lymph node histocultures. This year we further characterized the histoculture system. Pathogenic isolates of ADV can replicate in the cultures and produce infectious virus.We also studied outbreaks of ADV-induced neurological disease in ferrets and in mink. In both instances, the CNS tissue lesions were plasmacytic meningoencephalitis, suggesting an immunological basis to the disorder. ADV DNA was identified and sequenced. The sequence of the ferret ADV was similar to other isolates of ferret ADV, but the ADV from the mink encephalitis outbreak (ADV-TH) had several unique residues in the region of the capsid gene that governs pathogenicity. These studies indicate that ADV can cause immune mediated lesions in the nervous system. We do not yet know if this is a consequence of the virus isolate or the host response. - Parvoviruses, viral pathogenesis, lymphoid tissues, parvovirus B19, Aleutian mink disease parvovirus

成年水貂持续感染阿留申水貂病细小病毒（ADV）会导致免疫调节紊乱，包括高丙种球蛋白血症、浆细胞增多症、免疫复合物疾病、间质性肾炎和肾小球肾炎。极高水平的抗病毒抗体无法消除病毒，事实上反而会增强巨噬细胞等 Fc 受体细胞的感染。我们正在体内、体外和凝胶支持的淋巴结组织培养的离体系统中研究 ADV 感染。今年我们进一步表征了组织培养系统。 ADV 的致病分离株可以在培养物中复制并产生传染性病毒。我们还研究了 ADV 诱发的雪貂和水貂神经系统疾病的爆发。在这两种情况下，中枢神经系统组织病变均为浆细胞性脑膜脑炎，表明该疾病具有免疫学基础。 ADV DNA 被鉴定并测序。雪貂 ADV 的序列与雪貂 ADV 的其他分离株相似，但来自水貂脑炎暴发 (ADV-TH) 的 ADV 在控制致病性的衣壳基因区域有几个独特的残基。这些研究表明，ADV 可引起神经系统免疫介导的病变。我们尚不知道这是否是病毒分离或宿主反应的结果。 - 细小病毒、病毒发病机制、淋巴组织、细小病毒 B19、阿留申水貂病细小病毒