SPATIALLY SELECTIVE COAGULATION OF HYPERVASCULAR LESIONS

高血管病变的空间选择性凝固

• 批准号: 6266300
• 负责人: BAHMAN ANVARI
• 金额: $ 22.84万
• 依托单位: RICE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-03-19 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6266300
• 关键词: blood vessel disorder; body temperature; cardiovascular disorder therapy; chickens; clinical trials; cold temperature; combination therapy; cryoprotective agents; cryoscience; disease /disorder model; drug screening /evaluation; histology; human subject; human therapy evaluation; lasers; mathematical model; model design /development; nonhuman therapy evaluation; patient oriented research; photocoagulation therapy; radiation dosage; skin disorder; skin disorder chemotherapy; thermodynamics; whole body irradiation dosage

DESCRIPTION (Verbatim from Applicant's Abstract): Laser treatment of hypervascular cutaneous malformations such as port wine stain (PWS) lesion is based or selective photocoagulation of subsurface targeted blood vessels without inducing thermal damage to the superficial skin layers. The principal investigator and his collaborators are interested in development and optimization of cryogen spray cooling (CSC) to protect the superficial skin layers from thermal injury during high laser light dosages (2 10 J.cm about ), and long pulses (> 0.5 ms) for more efficient and effective clinical management of PWS patients. for more efficient and effective clinical management of PWS patients. To fully explore the potentials of CSC and optimize its efficiency under the most severe laser irradiation conditions that may be required for more effective treatment protocols, further studies aimed at understanding the thermodynamics and heat transfer processes associated with CSC are required. The specific objectives of this proposal are to: ( I ) develop mathematical models to predict skin internal temperature distributions following cryogen spurt termination by solving an inverse heat transfer formulation with a one dimensional inversion algorithm and subsequent, computation of temperature profiles in response to high incident light dosages and long laser pulses when specifically utilized in conjunction with CSC; (2) utilize various cryogen delivery s' about'ems to produce a wide range of droplet size, and study the subsequent thermal response of the substrate (i.e., an in-vitro epoxy resin model, and ex-vivo human skin samples, (3) conduct CSC and laser irradiation of ex-vivo human skin samples to determine optimum cooling parameters for epidermal protection under high incident light dosages and long pulse durations, and validate results of the mathematical modeling; (4) perform in-vivo experiments using the chicken comb animal model to investigate the utility of CSC in protecting the superficial tissue layers while allowing laser induced photocoagulation of dermal blood vessels; (5) utilize the information obtained from the aforementioned thermodynamic studies at theoretical, in-vitro, ex-vivo, and in-vivo animal model levels in conjunction with one-dimensional inversion of pulsed photothermal radiometry (PPTR) data to "recommend" optimum CSC and laser irradiation parameters for a specific lesion and skin type; and (6) Conduct clinical studies in PWS patients to evaluate the effectiveness of CSC in conjunction with laser irradiation using high incident dosage, longer pulses and wavelengths, and correlate the therapeutic outcome with the "recommended CSC and irradiation parameters."

描述（逐字病来自申请人的摘要）：激光处理 高血管皮肤畸形，例如港口葡萄酒染色（PWS）病变是 基于地下靶向血管的基于或选择性的光凝 不诱导对浅表皮层的热损害。校长 调查员及其合作者对发展感兴趣 优化低温喷雾冷却（CSC）以保护浅表皮肤 高激光剂量（大约2 10 j.cm）中的热损伤层， 和长脉冲（> 0.5 ms），以提高高效和有效的临床管理 PWS患者。为PWS的更有效和有效的临床管理 患者。充分探索CSC的潜力并优化其效率 在最严重的激光照射条件下 更有效的治疗方案，旨在了解 需要与CSC相关的热力学和传热过程。 该建议的具体目标是：（i）发展数学 预测低温后皮肤内部温度分布的模型 通过求解一个反向传热配方的终止 尺寸反演算法以及随后的温度计算 响应高入射光剂量和长激光脉冲时的轮廓 专门与CSC结合使用； （2）利用各种低温 交付s'关于'ems以产生各种液滴尺寸，并研究 随后的底物热响应（即，体外环氧树脂 模型和前体皮肤样品，（3）进行CSC和激光照射 体内人类皮肤样品以确定最佳冷却参数 高入射光剂量和长脉冲下的表皮保护 持续时间并验证数学建模的结果； （4）执行 使用鸡梳动物模型研究的体内实验 CSC在保护浅层组织层的同时允许激光的效用 诱导皮肤血管的光凝； （5）利用信息 从上述的热力学研究中获得的理论， 体外，前体和体内动物模型水平与结合 脉冲光热辐射测定法（PPTR）数据的一维反转至 “推荐”特定病变的最佳CSC和激光辐照参数 和皮肤类型； （6）对PWS患者进行临床研究以评估 使用高入射的CSC与激光照射结合的有效性 剂量，更长的脉冲和波长，并将治疗结果相关 使用“推荐的CSC和辐照参数”。