FRAMESHIFT REGULATION OF E COLI RNA POLYMERASE GENES

大肠杆菌 RNA 聚合酶基因的移码调控

基本信息

批准号：
6226480
负责人：
James F. CURRAN
金额：
$ 14.06万
依托单位：
WAKE FOREST UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-06-01 至 2004-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6226480
关键词：
DNA directed RNA polymerase Escherichia coli bacterial genetics bacterial proteins beta galactosidase enzyme activity genetic regulation microorganism culture microorganism growth nucleic acid sequence

项目摘要

DESCRIPTION: Proposed is a study of what may be a novel set of properties for the E. coli RNA polymerase core protein genes (rpoA, B and C genes). These genes are known to be growth rate regulated, and that regulation occurs by several transcriptional and translational mechanisms, but the detailed mechanisms and their coordination are largely obscure. Our sequence analyses show that the 5' ends of these genes contain UUU-Ynn dicodons, which are known to be highly frameshift-prone, and which are strongly avoided in other highly expressed genes. That these UUU-Ynn sequences occur near the 5' ends of all three rpo genes, and that this pattern is shared by diverse bacteria, suggests that these sites may have undiscovered, important functions. We will determine whether these sequences are frameshift-prone and whether frameshifting can contribute to regulation of these genes. The rpoB gene may be especially interesting because it encodes a potential frameshift-polypeptide that would be large enough to have function. This feature is also conserved among diverse bacteria. We will determine whether expression of this polypeptide has any global effect on cell physiology, and whether it specifically affects rpo gene expression. These studies may uncover interesting phenomena in the regulation and expression of the physiologically important rpo genes. This work will also train undergraduates interested in biomedical careers.

描述：提出了一项关于可能是一组新颖属性的研究大肠杆菌 RNA 聚合酶核心蛋白基因（rpoA、B 和 C 基因）。这些众所周知，基因是受生长速率调节的，并且这种调节是通过以下方式发生的：几种转录和翻译机制，但详细机制及其协调在很大程度上是模糊的。我们的序列分析显示这些基因的 5' 末端含有已知的 UUU-Ynn 双密码子高度移码倾向，并且在其他高度移码中强烈避免表达的基因。这些 UUU-Ynn 序列出现在所有序列的 5' 端附近三个 rpo 基因，并且这种模式是多种细菌共有的，表明这些站点可能具有未被发现的重要功能。我们将确定这些序列是否容易发生移码以及移码是否可以有助于这些基因的调节。 rpoB 基因可能特别有趣的是，它编码了一种潜在的移码多肽，即足够大，有功能。这一特征在不同的细菌。我们将确定该多肽的表达是否有任何影响对细胞生理学的整体影响，以及它是否特别影响 rpo 基因表达。这些研究可能会揭示监管中有趣的现象以及生理上重要的 rpo 基因的表达。这部作品也将培养对生物医学职业感兴趣的本科生。