ELF ELECTROMAGNETIC FIELDS AND CANCER

极低频电磁场与癌症

• 批准号: 6173146
• 负责人: HOWARD R PETTY
• 金额: $ 17.29万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-17 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6173146
• 关键词: DNA damage; NAD(H) phosphate; biophysics; cell adhesion; cell line; cell migration; cytoskeleton; electromagnetic radiation; electrophysiology; environment related neoplasm /cancer; environmental radiation; exo alpha sialidase; fluorescence spectrometry; free radical oxygen; hydrogen peroxide; hydroxyl radical; leukocytes; neoplasm /cancer invasiveness; neoplastic cell; nucleotidyltransferase; oxidative stress; proteolysis; radiation genetics; radiation related neoplasm /cancer; superoxides

DESCRIPTION: Epidemiological studies have variously associated extremely low frequency (ELF) electromagnetic fields with leukemia, brain and breast cancer. Unfortunately, a rational scientific mechanism linking such fields with cancer is not known. Thus, one long-range goal is to establish a mechanistic link between ELF fields and cancer. Recently, we have shown the metabolic oscillations of human cells can resonate during migration or adherence in the presence of phase-matched ELF electric fields within the environmentally relevant intensity region. Environmental electromagnetic subharmonics that match metabolic oscillation frequencies could induce metabolic resonance thereby dramatically altering normal oscillatory metabolic reactions and, consequently, cell morphology and function (cell migration/invasive/adherence properties). To test if metabolic resonance provides a mechanistic link betwee ELF fields and cancer, we will expose tumor cells, leukemic cells, and control to electric fields during migration, adherence and invasive activities (models of cell detachment, invasion and metastasis). To control for the phase of metabolic oscillations, we will monitor NAD (P) H autofluorescence in (resonance) and phase-mismatched conditions. We will characterize the electric field intensity dependence of metabolic resonance. We will also examine cell morphology, cytoskeletal changes and pericellular proteolysis during metabolic resonance. Our electromechanical coupling hypothesis may be a physical mechanism linking weak fields to metabolic resonance and its downstream bioeffects. To test this primary coupling mechanism we will test the role of cell surface charges in metabolic resonance. Preliminary data show that high-amplitude NAD (P) H oscillations are associated with superoxide anion production. We will study the electric field intensity and phase-dependence of reactive oxygen metabolite production within leukocytes, tumor and leukemic cells. Using the comet and terminal deoxynucleotidyl transferase assays, we will link metabolic resonance/oxidant stress with DNA damage. A mechanistic series of events linking ELF fields with adherence/locomotion/invasive properties of cancer cells, metabolism and DNA damage will be identified; this may provide a physicochemical basis for ELF health effects.

描述：流行病学研究极为相关 低频（ELF）电磁场，白血病，大脑和乳房 癌症。 不幸的是，将这些领域联系起来的理性科学机制 癌症是不知道的。 因此，一个远程目标是建立一个 精灵领域与癌症之间的机械联系。 最近，我们显示了 人类细胞的代谢振荡在迁移期间会产生共鸣或 在存在相匹配的精灵电场的情况下依从性 与环境相关的强度区域。 环境电磁 匹配代谢振荡频率的亚肝素可能会诱导 代谢共振，从而极大地改变了正常振荡 代谢反应，因此是细胞的形态和功能（细胞 迁移/侵入性/依从性属性）。 测试是否代谢共振 提供了一个机械链接，在小精灵领域和癌症之间，我们将展示 肿瘤细胞，白血病细胞以及对电场的控制 迁移，依从性和侵入性活动（细胞脱离模型， 入侵和转移）。 控制代谢阶段 振荡，我们将在（共振）和 相位不匹配的条件。 我们将表征电场 代谢共振的强度依赖性。 我们还将检查细胞 形态，细胞骨架变化和周围的细胞蛋白水解 代谢共振。 我们的机电耦合假设可能是 将弱领域与代谢共振及其它的物理机制联系起来 下游生物效应。 为了测试这种主要耦合机制，我们将测试 细胞表面电荷在代谢共振中的作用。 初步数据 表明高振幅NAD（P）H振荡与 超氧阴离子的产生。 我们将研究电场强度和 活性氧代谢物在白细胞中产生的相位依赖性， 肿瘤和白血病细胞。 使用彗星和末端脱氧核苷酸 转移酶测定法，我们将将代谢共振/氧化应激与DNA联系起来 损害。 将小精灵领域与 癌细胞，代谢和DNA的依从性/运动/侵入性特性 将确定损坏；这可能为精灵提供物理化学基础 健康影响。